Cysteine proteinase type I, encapsulated in solid lipid nanoparticles induces substantial protection against Leishmania major infection in C57BL/6 mice
| Autor: | Doroud, Delaram, Zahedifard, Farnaz, Vatanara, Alireza, Rouholamini Najafabadi, Abdolhossein, Rafati, Sima |
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| Přispěvatelé: | Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Institut Pasteur d'Iran, Réseau International des Instituts Pasteur (RIIP) |
| Rok vydání: | 2011 |
| Předmět: |
Protozoan Vaccines
Drug Carriers cysteine proteinase type I solid lipid nanoparticle Leishmaniasis Cutaneous vaccination delivery systems Mice Inbred C57BL Interferon-gamma Mice Adjuvants Immunologic Cysteine Proteases Immunoglobulin G Liposomes Leukocytes Mononuclear Animals Nanoparticles Female Interleukin-4 Lymph Nodes [SDV.IMM.VAC]Life Sciences [q-bio]/Immunology/Vaccinology Leishmania major |
| Zdroj: | Parasite Immunology Parasite Immunology, Wiley, 2011, 33 (6), pp.335-48. ⟨10.1111/j.1365-3024.2011.01289.x⟩ |
| ISSN: | 1365-3024 0141-9838 |
| DOI: | 10.1111/j.1365-3024.2011.01289.x⟩ |
| Popis: | International audience; Appropriate adjuvant, proper antigen(s) and a suitable formulation are required to develop stable, safe and immunogenic vaccines. Leishmanial cysteine proteinase type I (CPB) is a promising vaccine candidate; nevertheless, it requires a delivery system to induce a potent immune response. Herein, solid lipid nanoparticles (SLN) have been applied for CPB [with and without C-terminal extension (CTE)] formulation to utilize as a vaccine against Leishmania major infection in C57BL/6 mice. Therefore, SLN-CPB and SLN-CPB(-CTE) formulations were prepared from cetyl palmitate and cholesterol, using melt emulsification method. After intraperitoneal vaccination and subsequent L. major challenge, a strong antigen-specific T-helper type 1 (Th1) immune response was induced compared to control groups. Lymph node cells from immunized mice displayed lower parasite burden, higher IFN-γ, IgG2a and lower IL-4 production, indicating that robust Th1 immune response had been induced. Our results revealed that CTE is not necessary for inducing protective responses against L. major infection as the IFN-γ/IL-4 ratio was significantly higher, whereas IgG1 responses were lower in the SLN-CPB(-CTE) vaccinated group, post-challenge. Thus, SLN-CPB(-CTE) was shown to induce specific Th1 immune responses to control L. major infection, through effective antigen delivery to the peritoneal antigen presenting cells. |
| Databáze: | OpenAIRE |
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