Generation of live offspring from vitrified embryos with synthetic polymers Supercool X-1000 and Supercool Z-1000
| Autor: | Marco Jiménez, Francisco, Jiménez Trigos, María Estrella, Lavara García, Raquel, Vicente Antón, José Salvador |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Glycerol
Ethylene Glycol Ice blockers Polymers Embryonic Development Rabbit Mucin coat Buffers PRODUCCION ANIMAL Offspring Cryoprotective Agents In vitro Pregnancy In vivo Animals Dimethyl Sulfoxide Edetic Acid Cryopreservation Ice Embryo Transfer Embryo Mammalian Vitrification Polyvinyl Alcohol Female Polyvinyls Rabbits |
| Zdroj: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname Europe PubMed Central |
| Popis: | [EN] BACKGROUND: Ice growth and recrystallisation are considered important factors in determining vitrification outcomes. Synthetic polymers inhibit ice formation during cooling or warming of the vitrification process. OBJECTIVE: The aim of this study was to assess the effect of adding commercially available synthetic polymers SuperCool X-1000 and SuperCool Z-1000 to vitrification media on in vivo development competence of rabbit embryos. METHODS: Four hundred and thirty morphologically normal embryos recovered at 72 h of gestation were used. The vitrification media contained 20% dimethyl sulphoxide and 20% ethylene glycol, either alone or in combination with 1% of SuperCool X-1000 and 1% SuperCool. RESULT: Our results show that embryos can be successfully vitrified using SuperCool X-1000 and SuperCool Z-1000 and when embryos are transferred, live offspring can be successfully produced. CONCLUSIONS: In conclusion, our results demonstrated that we succeeded for the first time in obtaining live offspring after vitrification of embryos using SuperCool X-1000 and SuperCool Z-1000 polymers. This work was supported by the Spanish Research Project AGL2011-30170-C02-01 and by funds from the Generalitat Valenciana Research Programme (Prometeo 2009⁄125). Estrella Jiménez was supported by a research grant from the Education Ministry of the Valencian Regional Government (programme VALi+d. ACIF/2010/262). |
| Databáze: | OpenAIRE |
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