Apolipoprotein E pathology in vascular dementia
| Autor: | Rohn, Troy T, Day, Ryan J, Sheffield, Colin B, Rajic, Alexander J, Poon, Wayne W |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Aged
80 and over Male Dementia Vascular astrocytes Life Sciences Neurofibrillary Tangles Immunohistochemistry Vascular dementia plaques apoE Apolipoproteins E neurofibrillary tangles immunohistochemistry Medicine and Health Sciences Humans Original Article Female lipids (amino acids peptides and proteins) PHF-1 Aged apolipoprotein E |
| Zdroj: | Rohn, Troy T; Day, Ryan J; Sheffield, Colin B; Rajic, Alexander J; & Poon, Wayne W. (2014). Apolipoprotein E pathology in vascular dementia. International Journal of Clinical and Experimental Pathology, 7(3), 938-947. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/6hc0c7w7 |
| Popis: | Vascular dementia (VaD) is the second most common form of dementia and is currently defined as a cerebral vessel vascular disease leading to ischemic episodes. Apolipoprotein E (apoE) gene polymorphism has been proposed as a risk factor for VaD, however, to date there are few documented post-mortem studies on apoE pathology in the VaD brain. To investigate a potential role for the apoE protein, we analyzed seven confirmed cases of VaD by immunohistochemistry utilizing an antibody that specifically detects the amino-terminal fragment of apoE. Application of this antibody, termed N-terminal, apoE cleavage fragment (nApoECF) revealed consistent labeling within neurofibrillary tangles (NFTs), blood vessels, and reactive astrocytes. Labeling occurred in VaD cases that had confirmed APOE genotypes of 3/3, 3/4, and 4/4, with respect to NFTs, staining of the nApoECF co-localized with PHF-1 and was predominantly localized to large, stellate neurons in layer II of the entorhinal cortex. Quantitative analysis indicated that approximately 38.4% of all identified NFTs contained the amino-terminal fragment of apoE. Collectively, these data support a role for the proteolytic cleavage of apoE in the VaD and support previous reports that APOE polymorphism is significantly associated with susceptibility in this disease. |
| Databáze: | OpenAIRE |
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