メトホルミンは、CD90陽性筋線維芽細胞によって引き起こされる細胞外マトリックス産生を阻害することにより胸膜線維弾性症を軽減する

Autor: Aoshima, Yoichiro, Enomoto, Yasunori, Fukada, Atsuki, Kurita, Yuki, Matsushima, Sayomi, Meguro, Shiori, Kosugi, Isao, Kawasaki, Hideya, Katsura, Hiroaki, Fujisawa, Tomoyuki, Enomoto, Noriyuki, Nakamura, Yutaro, Inui, Naoki, Suda, Takafumi, Iwashita, Toshihide
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Am J Transl Res
ISSN: 1943-8141
Popis: Metformin, an AMP-activated protein kinase activator used to treat diabetes mellitus, has recently attracted attention as a promising anti-fibrotic agent. However, its anti-fibrotic effects on pleural fibroelastosis remain unknown. We induced mouse pleural fibroelastosis by intra-pleural coadministration of bleomycin and carbon and evaluated its validity as a preclinical model for human pleural fibrosis. We assessed the expression of the myofibroblast surface marker CD90 in the fibrotic pleura and the effects of metformin in vivo and in vitro. Finally, we evaluated the effects of metformin on human pleural mesothelial cells stimulated by transforming growth factor β1 (TGFβ1). The fibrotic pleura in mice had collagen and elastin fiber deposition similar to that seen in human fibrotic pleura. Moreover, CD90-positive myofibroblasts were detected in and successfully isolated from the fibrotic pleura. Metformin significantly suppressed the deposition of collagen and elastic fibers in the fibrotic pleura and decreased the expression of extracellular matrix (ECM)-related genes, including Col1a1, Col3a1, Fn1, and Eln, in pleural CD90-positive myofibroblasts. In human pleural mesothelial cells, metformin decreased TGFβ1-induced upregulation of ECM-related genes and SNAI1. Overall, metformin suppresses pleural fibroelastosis by inhibition of ECM production by pleural myofibroblasts, suggesting that this drug has therapeutic potential against human pleural fibrosis, including pleuroparenchymal fibroelastosis.
doctoral
医学系研究科
甲第894号
Databáze: OpenAIRE
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