Differentiation of mouse embryonic stem cells into endothelial cells: genetic selection and potential use in vivo
| Autor: | Gimond , Clotilde, Marchetti , Sandrine, Pagès , Gilles |
|---|---|
| Přispěvatelé: | Institut de signalisation, biologie du développement et cancer (ISBDC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Institut de signalisation, biologie du développement et cancer ( ISBDC ), Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ) |
| Rok vydání: | 2006 |
| Předmět: |
MESH: Cell Differentiation
MESH : Cell Culture Techniques Cell Transplantation Green Fluorescent Proteins Cell Culture Techniques Mice Nude [SDV.CAN]Life Sciences [q-bio]/Cancer [ SDV.BBM.BM ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology MESH: Stem Cells MESH: Extracellular Matrix MESH : Green Fluorescent Proteins [ SDV.CAN ] Life Sciences [q-bio]/Cancer Mice MESH: Green Fluorescent Proteins MESH: Reverse Transcriptase Polymerase Chain Reaction MESH : Mice MESH : Cells Cultured MESH: Mice Nude MESH : Embryo Mammalian Animals Humans MESH: Animals MESH: Endothelial Cells MESH: Mice Cells Cultured MESH: Cell Culture Techniques MESH: Humans MESH : Endothelial Cells Reverse Transcriptase Polymerase Chain Reaction Stem Cells MESH : Humans MESH : Reverse Transcriptase Polymerase Chain Reaction MESH : Extracellular Matrix MESH: Embryo Mammalian MESH : Mice Nude Endothelial Cells MESH: Antigens CD31 [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Cell Differentiation Embryo Mammalian MESH : Cell Transplantation Extracellular Matrix Platelet Endothelial Cell Adhesion Molecule-1 MESH: Cell Transplantation MESH : Antigens CD31 MESH : Stem Cells MESH : Animals MESH : Cell Differentiation MESH: Cells Cultured |
| Zdroj: | Methods in Molecular Biology Methods in Molecular Biology, Humana Press/Springer Imprint, 2006, 330, pp.303-29. ⟨10.1385/1-59745-036-7:303⟩ Methods in Molecular Biology (Clifton then Totova) Methods in Molecular Biology (Clifton then Totova), Humana Press (Springer Imprint), 2006, 330, pp.303-29. 〈10.1385/1-59745-036-7:303〉 |
| ISSN: | 1064-3745 1940-6029 |
| DOI: | 10.1385/1-59745-036-7:303⟩ |
| Popis: | International audience; Embryonic stem (ES) cells are pluripotent cells derived from blastocyst-stage embryos. They are characterized by their infinite self-renewal capacity and their ability to differentiate into many cell types in vitro as well as in vivo. The present protocol describes culture conditions that allow efficient differentiation of mouse ES cells toward the endothelial lineage, both in two-dimensional cultures and in three-dimensional, multicellular embryoid bodies. We also provide a protocol for establishing recombinant ES cell clones, giving the example of cells expressing green fluorescent protein and an antibiotic resistance gene under the control of an endothelial-specific promoter. These transgenes allow the visualization and the genetic selection of endothelial cells from a heterogeneous population of differentiating ES cells. Potential applications for these differentiation models are given, including the study of the endothelial cell lineage and its derivatives, the testing of molecules involved in angiogenesis, and the potential use of selected endothelial cells in vivo. |
| Databáze: | OpenAIRE |
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