Doxycycline inducible melanogenic vaccinia virus as theranostic anti-cancer agent
| Autor: | Kirscher, Lorenz, Deán-Ben, Xosé Luis, Scadeng, Miriam, Zaremba, Angelika, Zhang, Qian, Kober, Christina, Fehm, Thomas Felix, Razansky, Daniel, Ntziachristos, Vasilis, Stritzker, Jochen, Szalay, Aladar A. |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Gene Expression Regulation
Viral Male viruses Genetic Vectors Mice Nude Vaccinia virus Oncolysis Theranostic Nanomedicine Mice Molecular Imaging Reporter Gene Virotherapy Cell Line Tumor Neoplasms Animals Humans ddc:610 Melanins Oncolytic Virotherapy Monophenol Monooxygenase Magnetic Resonance Imaging Reporter gene Tumor Burden Radiography Doxycycline virotherapy Research Paper |
| Zdroj: | Theranostics 5, 1045-1057 (2015) Theranostics |
| Popis: | We reported earlier the diagnostic potential of a melanogenic vaccinia virus based system in magnetic resonance (MRI) and optoacoustic deep tissue imaging (MSOT). Since melanin overproduction lead to attenuated virus replication, we constructed a novel recombinant vaccinia virus strain (rVACV), GLV-1h462, which expressed the key enzyme of melanogenesis (tyrosinase) under the control of an inducible promoter-system. In this study melanin production was detected after exogenous addition of doxycycline in two different tumor xenograft mouse models. Furthermore, it was confirmed that this novel vaccinia virus strain still facilitated signal enhancement as detected by MRI and optoacoustic tomography. At the same time we demonstrated an enhanced oncolytic potential compared to the constitutively melanin synthesizing rVACV system. |
| Databáze: | OpenAIRE |
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