Localization, Characterization, and Second Messenger Coupling of Pituitary Adenylate Cyclase-Activating Polypeptide Receptors in the Fetal Human Adrenal Gland during the Second Trimester of Gestation*
| Autor: | L, Yon, L, Breault, V, Contesse, G, Bellancourt, C, Delarue, A, Fournier, J G, Lehoux, H, Vaudry, N, Gallo-Payet |
|---|---|
| Přispěvatelé: | Neuroendocrinologie cellulaire et moléculaire, Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Fédératif de Recherches Multidisciplinaires sur les Peptides (IFRMP 23), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Lutte Contre le Cancer Henri Becquerel Normandie Rouen (CLCC Henri Becquerel)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-CHU Rouen, Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Faculté de médecine et des sciences de la santé [Sherbrooke] (UdeS), Université de Sherbrooke (UdeS), Université du Québec à Montréal = University of Québec in Montréal (UQAM), Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique [Québec] (INRS)-Réseau International des Instituts Pasteur (RIIP), This work was supported by the Medical Research Council of Canada (MRC MT13679, to N.G.-P. and J.-G.L.), the Institut National de la Santé et de la Recherche Médicale (INSERM U413), a France-Québec exchange programme (Coopération Scientifique et Technologique Franco-Québécoise (PVP-73–9, to N.G.-P. and H.V.), and the Conseil Régional de Haute-Normandie., The authors would like to thank Ms. Huguette Lemonnier (INSERM U413) and Lucie Chouinard (Service of Endocrinology, Sherbrooke) for skillful technical assistance. We appreciate the advice from Drs. Magali Basille and Bruno Gonzalez (INSERM U413) for autoradiography. |
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
MESH: Receptors
Pituitary Hormone/metabolism [SDV]Life Sciences [q-bio] Receptors Pituitary Adenylate Cyclase-Activating Polypeptide MESH: Logistic Models Second Messenger Systems Embryonic and Fetal Development MESH: Autoradiography MESH: Pregnancy Pregnancy MESH: Analysis of Variance Adrenal Glands Humans Receptors Pituitary Hormone Cells Cultured MESH: Embryonic and Fetal Development/physiology Analysis of Variance MESH: Receptors Pituitary Adenylate Cyclase-Activating Polypeptide MESH: Second Messenger Systems/physiology MESH: Humans MESH: Adrenal Glands/metabolism MESH: Adrenal Glands/embryology Logistic Models Pregnancy Trimester Second MESH: Pregnancy Trimester Second MESH: Receptors Pituitary Adenylate Cyclase-Activating Polypeptide Type I Autoradiography Female MESH: Female Receptors Pituitary Adenylate Cyclase-Activating Polypeptide Type I MESH: Cells Cultured |
| Zdroj: | The Journal of clinical endocrinology and metabolism The Journal of clinical endocrinology and metabolism, Williams & Wilkins Co., 1997, 83 (4), pp.1299-1305. ⟨10.1210/jcem.83.4.4690⟩ Journal of Clinical Endocrinology and Metabolism Journal of Clinical Endocrinology and Metabolism, Endocrine Society, 1998, 83 (4), pp.1299-1305. ⟨10.1210/jcem.83.4.4690⟩ |
| ISSN: | 0021-972X 1945-7197 |
| DOI: | 10.1210/jcem.83.4.4690⟩ |
| Popis: | International audience; The distribution and pharmacological properties of pituitary ad-enylate cyclase-activating polypeptide (PACAP) receptors were studied in the fetal human adrenal gland during the second trimester of gestation. Autoradiographic studies, using [ 125 I]PACAP27 as a ra-dioligand, revealed that PACAP-binding sites are exclusively located on chromaffin cells of adrenals from fetuses 14 –20 weeks old. Biochemical characterization of binding revealed the occurrence of a single class of PACAP-binding sites with a dissociation constant value of 0.32– 0.74 nmol/L and a binding capacity of 0.30 – 0.81 pmol/mg wet tissue. PACAP27 and PACAP38 were equipotent in competing for [ 125 I]PACAP27 binding (IC 50 0.28 – 0.64 nmol/L and 0.15– 0.81 nmol/L, respectively), and the Hill coefficients were close to 1. In contrast, vasoactive intestinal polypeptide was much less efficient in displacing the tracer (IC 50 4 –362 nmol/L), and the Hill coefficients were less than 0.6. PACAP38 induced a dose-dependent increase in cAMP production in fetal human adrenal cell suspension (ED 50 0.07 0.02 nmol/L), as well as in cells maintained in culture for 5 days (5.4 1.8 nmol/L). In constrast, PACAP38 induced a modest increase in inositol phosphate formation. These data indicate that type I PACAP receptors are present in the early stages of the human me-dulla organization during the process of migration of chromaffin cells from the periphery to the central part of the gland. The present results suggest that PACAP could be involved in the regulation of the human adrenochromaffin cells during ontogenesis. (J Clin Endocrinol Metab 83: 1299 –1305, 1998) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|