Salvianolic Acid B-Loaded Chitosan/hydroxyapatite Scaffolds Promotes The Repair Of Segmental Bone Defect By Angiogenesis And Osteogenesis
| Autor: | Ji,Chuanlei, Bi,Long, Li,Jing, Fan,Junjun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Vascular Endothelial Growth Factor A
Neovascularization Physiologic Bone and Bones osteogenesis salvianolic acid B Cell Line angiogenesis Mice International Journal of Nanomedicine Spectroscopy Fourier Transform Infrared Human Umbilical Vein Endothelial Cells Animals Humans bone tissue engineering Original Research Benzofurans Cell Proliferation Chitosan Tissue Scaffolds drug-loaded bone scaffold Alkaline Phosphatase Drug Liberation Durapatite Rabbits controlled release Tomography X-Ray Computed |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 1176-9114 |
| Popis: | Chuanlei Ji,* Long Bi,* Jing Li, Junjun Fan Department of Orthopedics Surgery, Xijing Hospital, Air Force Medical University, Xi’an 710032, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jing Li; Junjun FanDepartment of Orthopedics Surgery, Xijing Hospital, Air Force Medical University, Xi’an 710032, People’s Republic of ChinaEmail zyfmmu@fmmu.edu.cn; fanjunjunys@163.comBackground: Salvianolic acid B has been proven as an effective drug to promote osteogenesis and angiogenesis which could be beneficial for bone repair.Purpose: The objective of this study was to construct a salvianolic acid B-loaded chitosan/hydroxyapatite (Sal B-CS/HA) bone scaffold with controlled release and effective bioactivity.Methods: The characteristics, controlled release behavior and bioactivity of Sal B-CS/HA scaffold were evaluated in vitro. The bone repair effect was evaluated in the rabbit radius defect model.Results: The results showed that chemical and physical characteristics of salvianolic acid B and chitosan/hydroxyapatite (CS/HA) material did not obviously change after the drug loading procedure; the drug release of salvianolic acid B was stable and continuous from the Sal B-CS/HA scaffold for 8 weeks in vitro; the biocompatibility of the Sal B-CS/HA was favorable by evaluation of cell morphology and proliferation; the osteogenic and angiogenic bioactivities of the Sal B-CS/HA scaffold were proved to be effective by in vivo and in vitro tests.Conclusion: Our results suggest that this salvianolic acid B-loaded bone scaffold has potential to be used for bone defect repair with both osteogenic and angiogenic bioactivities.Keywords: angiogenesis, bone tissue engineering, controlled release, drug-loaded bone scaffold, osteogenesis, salvianolic acid B |
| Databáze: | OpenAIRE |
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