Pneumocystis jirovecii genotyping: experience in a tertiary-care hospital in Northern Italy
| Autor: | Pietro, Pini, Carlotta F, Orsi, Annunziata, La Regina, Samuele, Peppoloni, Federica, Berrilli, Elisabetta, Blasi, David, Di Cave |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male Chronic Obstructive Genotype Genotyping Techniques Lymphoma RNA Mitochondrial Pilot Projects Pneumocystis jirovecii genotyping pneumonia Pneumocystis carinii Pulmonary Disease Tertiary Care Centers Pulmonary Disease Chronic Obstructive Genetic 80 and over Acquired Immunodeficiency Syndrome Aged Aged 80 and over Bronchoalveolar Lavage Fluid Female Humans Italy Middle Aged Pneumonia Pneumocystis RNA RNA Fungal Retrospective Studies Polymorphism Genetic Polymorphism Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali Pneumocystis Mitochondrial Fungal |
| Popis: | Respiratory samples from Pneumocystis jirovecii pneumonia (PJP) cases collected at a tertiary-care university hospital in Modena were analyzed for the presence of specific polymorphisms in the mitochondrial large subunit ribosomal RNA (mtLSU-rRNA). Retrospectively, 57 cases were selected in a six-year period and 34 out of the 57 processed BAL samples returned PCR positive results, thus allowing further molecular analysis. The following P.jirovecii genotype distribution was observed: genotype 3 (50%), genotype 2 (23%), genotype 1 (18%), genotypes 1 or 4 (9%). These data add novel insights on P.jirovecii epidemiology, investigating a previously unstudied area of Northern Italy. A peculiar local distribution is highlighted with respect to other areas within the national panorama, thus encouraging further in-depth studies in an attempt to better understand the overall situation concerning P.jirovecii genotype circulation. |
| Databáze: | OpenAIRE |
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