Nutrigenomic analyses reveal miRNAs and mRNAs affected by feed restriction in the mammary gland of midlactation dairy cows
| Autor: | Pierre-Alexis Billa, Yannick Faulconnier, Tao Ye, Céline Bourdon, José A A Pires, Christine Leroux |
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| Přispěvatelé: | Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Génétique Animale et Biologie Intégrative (GABI), Université Paris-Saclay-AgroParisTech-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Region Auvergne S3 23000794/1130, European Commission 23000794/1130, ApisGene, GISA meta-program of INRAE (Ruminflame project), GISA meta-program of INRAE (Longhealth project), INRAE, Université Clermont Auvergne, Vetagro Sup, UMR Herbivores, 63122 Saint-Genès-Champanelle, France |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Physiology
Microarrays [SDV]Life Sciences [q-bio] Gene Expression Biochemistry feed restriction cows Nutrigenomics Endocrinology Reproductive Physiology Medicine and Health Sciences Fatty Acids High-Throughput Nucleotide Sequencing food and beverages Lipids Body Fluids Nucleic acids Chemistry Milk Bioassays and Physiological Analysis Physical Sciences miRNAs Medicine Female Anatomy Research Article Science Mammary gland Research and Analysis Methods Beverages Mammary Glands Animal transcriptomes Genetics Animals Lactation RNA Messenger Lactic Acid Non-coding RNA Cell Proliferation Nutrition Natural antisense transcripts Biology and life sciences Endocrine Physiology Lipogenesis Chemical Compounds Lipid Metabolism Animal Feed Gene regulation Diet MicroRNAs Metabolism RNA Cattle Energy Metabolism Food Deprivation Transcriptome Acids |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2021, 16 (4), ⟨10.1371/journal.pone.0248680⟩ PLoS ONE, Public Library of Science, 2021, 16, ⟨10.1371/journal.pone.0248680⟩ PLoS ONE, Vol 16, Iss 4, p e0248680 (2021) |
| ISSN: | 1932-6203 |
| Popis: | International audience; The objective of this study was to investigate the effects of feed restriction on mammary miRNAs and coding gene expression in midlactation cows. Five Holstein cows and 6 Montbé liarde cows underwent 6 days of feed restriction, during which feed allowance was reduced to meet 50% of their net energy for lactation requirements. Mammary biopsies were performed before and at the end of the restriction period. Mammary miRNA and mRNA analyses were performed using high-throughput sequencing and microarray analyses, respectively. Feed restriction induced a negative energy balance and decreased milk production and fat and protein yields in both breeds. Feed restriction modified the expression of 27 miRNAs and 374 mRNAs in mammary glands from Holstein cows, whereas no significant miRNA change was observed in Montbé liarde cows. Among the 27 differentially expressed miRNAs, 8 miRNAs were associated with dairy QTL. Analysis of target genes indicate that the 8 most abundantly expressed miRNAs control transcripts related to lipid metabolism, mammary remodeling and stress response. A comparison between the mRNAs targeted by the 8 most strongly expressed miRNAs and 374 differentially expressed mRNAs identified 59 mRNAs in common. The bioinformatic analyses of these 59 mRNAs revealed their implication in lipid metabolism and endothelial cell proliferation. These effects of feed restriction on mammary miRNAs and mRNAs observed in Holstein cows suggest a potential role of miRNAs in mammary structure and lipid biosynthesis that could explain changes in milk production and composition. |
| Databáze: | OpenAIRE |
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