A Genotypic Comparison Reveals That the Improvement in Nitrogen Remobilization Efficiency in Oilseed Rape Leaves Is Related to Specific Patterns of Senescence-Associated Protease Activities and Phytohormones
| Autor: | Poret, Marine, Chandrasekar, Balakumaran, van der Hoorn, Renier A. L., Déchaumet, Sylvain, Bouchereau, Alain, Kim, Tae-Hwan, Lee, Bok-Rye, Macquart, Flavien, Hara-Nishimura, Ikuko, Avice, Jean-Christophe |
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| Přispěvatelé: | Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Department of economics, Yonsei University, Department of Metabolic Biology, John Innes Centre [Norwich], Kyoto University [Kyoto], ANR-11-BTBR-004 RAPSODYN – Investments for the Future: Optimisation of the RAPeSeed Oil content and Yield under low Nitrogen input), a Ph.D. grant from the French Ministry of Research, ERA-IB project 'PRODuCE', the Max Planck Society, COST CM1004 and the University of Oxford, Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Biotechnology and Biological Sciences Research Council (BBSRC)-Biotechnology and Biological Sciences Research Council (BBSRC), Kyoto University, ANR-11-BTBR-0004,RAPSODYN,Optimisation de la teneur et du rendement en huile chez le colza cultivé sous contrainte azotée(2011) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
azote
plante proteagineuse Vegetal Biology dégradation protéolytique nitrogen remobilization efficiency senescence food and beverages Brassica napus L regulation Plant Science sénescence foliaire phytohormones fertilisation azotée brassica napus l [SDV.BV]Life Sciences [q-bio]/Vegetal Biology protease activity recyclage de l'azote Biologie végétale remobilisation d'azote ComputingMilieux_MISCELLANEOUS Original Research |
| Zdroj: | Frontiers in Plant Science Frontiers in Plant Science, Frontiers, 2019, 10, pp.46. ⟨10.3389/fpls.2019.00046⟩ Frontiers in Plant Science (10), . (2019) Frontiers in Plant Science, 2019, 10, pp.46. ⟨10.3389/fpls.2019.00046⟩ |
| ISSN: | 1664-462X |
| DOI: | 10.3389/fpls.2019.00046⟩ |
| Popis: | Oilseed rape (Brassica napus L.) is an oleoproteaginous crop characterized by low N use efficiency (NUE) that is mainly related to a weak Nitrogen Remobilization Efficiency (NRE) during the sequential leaf senescence of the vegetative stages. Based on the hypothesis that proteolysis efficiency is crucial for the improvement of leafNRE, our objective was to characterize key senescence-associated proteolytic mechanisms of two genotypes (Ténor and Samouraï) previously identified with contrasting NREs. To reach this goal, biochemical changes, protease activities and phytohormone patterns were studied in mature leaves undergoing senescence in two genotypes with contrasting NRE cultivated in a greenhouse under limiting or ample nitrate supply. The genotype with the higher NRE (Ténor) possessed enhanced senescence processes in response to nitrate limitation, and this led to greater degradation of soluble proteins compared to the other genotype (Samouraï). This efficient proteolysis is associated with (i) an increase in serine and cysteine protease (CP) activities and (ii) the appearance of new CP activities (RD21-like, SAG12-like, RD19-like, cathepsin-B, XBCP3-like and aleurain-like proteases) during senescence induced by N limitation. Compared to Samouraï, Ténor has a higher hormonal ratio ([salicylic acid] + [abscisic acid])/([cytokinins]) that promotes senescence, particularly under low N conditions, and this is correlated with the stronger protein degradation and serine/CP activities observed during senescence. Short statement: The improvement in N recycling during leaf senescence in a genotype of Brassica napus L. characterized by a high nitrogen remobilization efficiency is related to a high phytohormonal ratio ([salicylic acid] + [abscisic acid])/([cytokinins]) that promotes leaf senescence and is correlated with an increase or the induction of specific serine and cysteine protease activities. |
| Databáze: | OpenAIRE |
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