Effects of cryoprotectant agents and equilibration methods on developmental competence of porcine oocytes

Autor:	Taniguchi, M., Arikawa, R., Kaedei, Y., Tanihara, F., Namula, Z., Viet, V. L., Sato, Y., Takeshige Otoi
Předmět:	Glycerol Male Ethylene Glycol Blastocyst Cryoprotective Agents Swine Oocytes Animals Embryonic Development Female Fertilization in Vitro Propylene Glycol Spermatozoa
Zdroj:	Scopus-Elsevier Europe PubMed Central
Popis:	Chemical toxicity of cryoprotectants to in vitro developmental competence of porcine oocytes was examined. In vitro-matured oocytes were exposed to 40 percent ethylene glycol (EG), glycerol (GLY), or 1,2-propanediol (PD), fertilized with spermatozoa, and cultured for 8 days. Compared to treatment with other cryoprotectants, exposure to EG resulted in the development of significantly more blastocysts, but the rate was significantly lower than that of non-exposed control oocytes. In vitro-matured oocytes were also equilibrated in 40 percent EG by 3 multi-step methods, after which their developmental competence was evaluated. The rate of blastocyst development was higher in the 4-step method than in the 2- and 3-step methods of equilibrium. These results indicate that cryoprotectants and equilibration methods affect the developmental competence of porcine oocytes and that EG may be a superior cryoprotectant for vitrification of these cells.
Databáze:	OpenAIRE
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