Forward motility is essential for trypanosome infection in the tsetse fly
| Autor: | Rotureau, Brice, Ooi, Cher-Pheng, Huet, Diego, Perrot, Sylvie, Bastin, Philippe |
|---|---|
| Přispěvatelé: | Biologie Cellulaire des Trypanosomes, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), ANR-08-MIEN-0027,SENSOTRYPA,Rôles sensoriels du flagelle au cours du cycle parasitaire du trypanosome africain(2008), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2013 |
| Předmět: |
Male
MESH: Locomotion/genetics Tsetse Flies DNAI1 MESH: RNA Interference Trypanosoma brucei brucei MESH: Gastrointestinal Tract/parasitology flagellum MESH: Digestive System/metabolism MESH: Trypanosoma brucei brucei/growth & development MESH: RNA Small Interfering MESH: Tsetse Flies/parasitology Animals MESH: Animals Trypanosoma brucei tsetse fly RNA Small Interfering MESH: Trypanosomiasis African/parasitology Dyneins Cell Differentiation MESH: Dyneins/genetics MESH: Cell Differentiation/genetics infection MESH: Male Gastrointestinal Tract [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Trypanosomiasis African motility RNA Interference vector Digestive System Locomotion |
| Zdroj: | Cellular Microbiology Cellular Microbiology, 2014, 16 (3), pp.425-433. ⟨10.1111/cmi.12230⟩ Cellular Microbiology, Wiley, 2014, 16 (3), pp.425-433. ⟨10.1111/cmi.12230⟩ |
| ISSN: | 1462-5822 1462-5814 |
| DOI: | 10.1111/cmi.12230⟩ |
| Popis: | International audience; African trypanosomes are flagellated protozoan parasites transmitted by the bite of tsetse flies and responsible for sleeping sickness in humans. Their complex development in the tsetse digestive tract requires several differentiation and migration steps that are thought to rely on trypanosome motility. We used a functional approach in vivo to demonstrate that motility impairment prevents trypanosomes from developing in their vector. Deletion of the outer dynein arm component DNAI1 results in strong motility defects but cells remain viable in culture. However, although these mutant trypanosomes could infect the tsetse fly midgut, they were neither able to reach the foregut nor able to differentiate into the next stage, thus failing to complete their parasite cycle. This is the first in vivo demonstration that trypanosome motility is essential for the accomplishment of the parasite cycle. |
| Databáze: | OpenAIRE |
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