Two Protocols to Study the Interactions of Thyroid Hormone Receptors with Other Proteins and Chromatin
| Autor: | Holbech, Henrik, Matthiessen, Peter, Hansen, Martin, Schüürmann, Gerrit, Knapen, Dries, Reuver, Marieke, Sachs, Laurent, Kloas, Werner, Hilscherova, Klara, Leonard, Marc, Arning, Jürgen, Strauss, Volker, Iguchi, Taisen, Baumann, Lisa, le Maire, Albane, Bouhours-Nouet, Natacha, Soamalala, Jessica, Mirebeau-Prunier, Delphine, Paloni, Matteo, Guee, Laura, Heron, Delphine, Mignot, Cyril, Illouz, Frédéric, Joubert, Florence, Briet, Claire, Rodien, Patrice, Bourguet, William, Sun, Hui, Wu, Haiying, Xie, Rongrong, Wang, Fengyun, Chen, Ting, Chen, Xiuli, Wang, Xiaoyan, Chen, Linqi, Guan, Wenyue, Guyot, Romain, Richard, Sabine, Flamant, Frederic |
|---|---|
| Přispěvatelé: | Institut de Génomique Fonctionnelle de Lyon (IGFL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Co-immunoprecipitation
placenta Chromatin affinity purification MESH: Carrier Proteins transporters integrated approach to testing and assessment MESH: Risk Assessment test guideline MESH: Chromatin MESH: Workflow genetic disease Animals Humans Immunoprecipitation MESH: Species Specificity MESH: Protein Binding nuclear receptor MESH: Animals MESH: Receptors Thyroid Hormone AOP [SDV.BDD]Life Sciences [q-bio]/Development Biology thyroid hormones Receptors Thyroid Hormone MESH: Humans neurodevelopment MESH: Immunoprecipitation biomarkers thyroid hormone disruption endocrine disruption thyroid hormone neurodevelopmental disorder Chromatin adverse outcome pathway Thyroid hormone receptors MESH: Health Plan Implementation OECD IATA Carrier Proteins cross-species extrapolation MESH: Health Impact Assessment Protein Binding brain–blood barrier |
| Zdroj: | Frontiers in Endocrinology Frontiers in Endocrinology, 2018, 1801 (3), pp.9-16. ⟨10.1007/978-1-4939-7902-8_2⟩ Frontiers in Endocrinology, Frontiers, 2018, 1801 (3), pp.9-16. ⟨10.1007/978-1-4939-7902-8_2⟩ |
| ISSN: | 1664-2392 |
| DOI: | 10.1007/978-1-4939-7902-8_2⟩ |
| Popis: | International audience; Understanding the transcriptional function of thyroid hormone receptors implies a precise analysis of their interactions with chromatin and other protein components of the cells. We present here two protocols that are routinely used in our laboratory. The first co-immunoprecipitation procedure allows addressing the capacity of proteins to form stable multiprotein complexes with TRs in cells. The chromatin affinity purification enables us to define the sites occupied by TRs on chromatin. In this case the lack of high quality antibodies is circumvented by introducing an N-terminal tag in TR, with unspecific affinity for immunoglobulins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|