| Autor: |
Carducci, C, Ellul, L, Antonozzi, I, Pontecorvi, Alfredo |
| Jazyk: |
angličtina |
| Rok vydání: |
1992 |
| Předmět: |
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| Popis: |
A quick, sensitive and easily automatizable method for PCR amplification of genomic DNA eluted from dried blood spots is described. DNA is eluted from a 3-mm spot routinely used for neonatal screening of inherited diseases either by boiling or by sonication. A preliminary and brief spot-autoclaving step is mandatory to ensure optimal and reproducible PCR amplifications. Only 1% of the eluted DNA is required for PCR analysis allowing the execution of multiple genetic tests on the same blood spot. The method has been successfully applied to the detection of a known phenylketonuria-causing mutation and will facilitate the analysis of the genetic repository provided by Guthrie's cards stored in neonatal screening laboratories. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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