High performance liquid chromatographic method for the determination of HIV-1 protease inhibitor tipranavir in plasma of patients during highly active antiretroviral therapy

Autor:	Langmann P, Werner Heinz, Klinker H, Schirmer D, Guhl C, Leyh M, Winzer R
Rok vydání:	2008
Předmět:	Sulfonamides Pyridines Pyrones Antiretroviral Therapy Highly Active HIV-1 Humans Reproducibility of Results HIV Infections HIV Protease Inhibitors Drug Monitoring Sensitivity and Specificity Chromatography High Pressure Liquid
Zdroj:	Europe PubMed Central
ISSN:	0949-2321
Popis:	A new high-performance liquid chromatographic method for the determination of tipranavir in human plasma is described. Quantitative recovery following liquid-liquid-extraction with diethylether from 100 microl of human plasma was achieved. Subsequently, the assay was performed with 67 mM potassium dihydrogen phosphate-acetonitrile as a mobile phase, a Phenomenex C 18 column and UV detection at 255 nm. Linear Standard curves were obtained for concentrations ranging from 2.5 to 400 microg/ml. The calculated intra- and inter-day coefficents of variation were below 7%.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::ceba68a9fce5f9ea6d6bf11e9d448495 https://pubmed.ncbi.nlm.nih.gov/18424362 Zobrazit plný text záznamu