Silencing of hSlo potassium channels in human osteosarcoma cells promotes tumorigenesis
| Autor: | Cambien, Béatrice, Rezzonico, Roger, Vitale, Sébastien, Rouzaire-Dubois, Béatrice, Dubois, Jean-Marc, Barthel, Robert, Karimdjee, Babou Soilihi, Mograbi, Baharia, Schmid-Alliana, Annie, Schmid-Antomarchi, Heidy |
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| Přispěvatelé: | Immunite anti-tumorale et chimiotactisme. Adenocarcinomes et métastases, Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-IFR50-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), Université Nice Sophia Antipolis - Faculté des Sciences (UNS UFR Sciences), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Institut de Neurobiologie Alfred Fessard (INAF), Centre National de la Recherche Scientifique (CNRS), Laboratoire de neurobiologie cellulaire et moléculaire (NBCM), Service de Chirurgie Générale et Cancérologie Digestive, Centre Hospitalier Universitaire de Nice (CHU Nice), Infection bactérienne, inflammation, et carcinogenèse digestive |
| Rok vydání: | 2008 |
| Předmět: |
MESH: Cell Line
Tumor MESH: Osteosarcoma MESH: RNA Interference MESH: Flow Cytometry Bone Neoplasms Enzyme-Linked Immunosorbent Assay MESH: Large-Conductance Calcium-Activated Potassium Channel alpha Subunits Polymerase Chain Reaction MESH: Plasmids Cell Line Tumor MESH: RNA Small Interfering MESH: Blotting Northern Humans RNA Small Interfering Large-Conductance Calcium-Activated Potassium Channel alpha Subunits Osteosarcoma MESH: Humans MESH: Enzyme-Linked Immunosorbent Assay MESH: Polymerase Chain Reaction MESH: Gene Expression Regulation Neoplastic MESH: Bone Neoplasms Blotting Northern Flow Cytometry Gene Expression Regulation Neoplastic [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] RNA Interference Plasmids |
| Zdroj: | International Journal of Cancer International Journal of Cancer, Wiley, 2008, 123 (2), pp.365-71. ⟨10.1002/ijc.23511⟩ |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.23511⟩ |
| Popis: | Erratum in: Int J Cancer. 2008 Sep 15;123(6):1481. Soilihi, Babou Karimdjee [corrected to Karimdjee, Babou Soilihi]; International audience; Potassium channels, the most diverse superfamily of ion channels, have recently emerged as regulators of carcinogenesis, thus introducing possible new therapeutic strategies in the fight against cancer. In particular, the large conductance Ca(2+)-activated K(+) channels, often referred to as BK channels, are at the crossroads of several tumor-associated processes such as cell proliferation, survival, secretion and migration. Despite the high BK channel expression in osteosarcoma (OS), their function has not yet been investigated in this malignant bone pathology. Here, using stable RNA interference to reduce the expression of hSlo, the human pore-forming alpha-subunit of the BK channel, in human Cal72 OS cells, we show that BK channels play a functional role in carcinogenesis. Our results reveal for the first time that BK channels exhibit antitumoral properties in OS in vivo and affect the tumor microenvironment through the modulation of both chemokine expression and leukocyte infiltration. |
| Databáze: | OpenAIRE |
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