Early expression of AMPA receptors and lack of NMDA receptors in developing rat climbing fibre synapses

Autor: Lachamp, Philippe, Balland, Bénedicte, Tell, Fabien, Baude, Agnès, Strube, Caroline, Crest, Marcel, Kessler, Jean-Pierre
Přispěvatelé: Laboratoire de neurophysiologie cellulaire (LNPC), Université de la Méditerranée - Aix-Marseille 2-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2005
Předmět:
Zdroj: The Journal of Physiology
The Journal of Physiology, Wiley, 2005, 564 (3), pp.751-763. ⟨10.1113/jphysiol.2005.084517⟩
The Journal of Physiology, 2005, 564 (3), pp.751-763. ⟨10.1113/jphysiol.2005.084517⟩
ISSN: 0022-3751
1469-7793
DOI: 10.1113/jphysiol.2005.084517⟩
Popis: Whether nascent glutamatergic synapses acquire their AMPA receptors constitutively or via a regulated pathway triggered by pre-existing NMDA receptor activation is still an open issue. Here, we provide evidence that some glutamatergic synapses develop without expressing NMDA receptors. Using immunocytochemistry, we showed that synapses between developing rat climbing fibres and Purkinje cells expressed GluR2-containing AMPA receptors as soon as they were formed (i.e. on embryonic day 19) but never carried detectable NMDA receptors. This was confirmed by electrophysiological recordings. Excitatory synaptic currents were recorded in Purkinje cells as early as P0. However, no NMDA receptor-mediated component was found in either spontaneous or evoked synaptic responses. In addition, we ruled out a possible role of extrasynaptic NMDA receptors by showing that AMPA receptor clustering at nascent climbing fibre synapses was not modified by chronic in utero NMDA receptor blockade.
Databáze: OpenAIRE
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