Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source
| Autor: | Kissenkötter, Jonas, Böhlken-Fascher, Susanne, Abd El Wahed, Ahmed |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Foods, Vol 9, Iss 1392, p 1392 (2020) Foods Volume 9 Issue 10 |
| ISSN: | 2304-8158 |
| Popis: | Detection of animal species in meat product is crucial to prevent adulterated and unnecessary contamination during processing, in addition to avoid allergy and religious consequences. Gold standard is the real-time PCR assays, which has a limited target capability. In this study, we have established a rapid sequencing protocol to identify animal species within hours. Sequencing was achieved by nanopore sequencing and data analysis via offline BLAST search. The whole procedure was conducted in a mobile suitcase lab. As per national and international regulations, the developed assay detected adulteration of pork meat with 0.1% of horse, chicken, turkey, cattle, sheep, duck, rabbit, goat, and donkey. The developed test could be used on-site as a rapid and mobile detection system to determine contamination of meat products Open-Access-Publikationsfonds 2020 peerReviewed |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|