Localization of the single copy gene Mdh2 on Xenopus tropicalis chromosomes by FISH-TSA

Autor:	Vladimir Krylov, Tlapakova T, Macha J
Rok vydání:	2006
Předmět:	DNA Complementary Sequence Homology Amino Acid Genetic Linkage Xenopus Molecular Sequence Data Chromosome Mapping Nucleic Acid Hybridization Xenopus Proteins Xenopus laevis Malate Dehydrogenase Animals Hybridization Genetic Amino Acid Sequence Cloning Molecular In Situ Hybridization Fluorescence
Zdroj:	Europe PubMed Central
ISSN:	1424-859X
Popis:	A single copy gene, mitochondrial malate dehydrogenase 2 (Mdh2), was localized on Xenopus tropicalis chromosomes by fluorescence in situ hybridization coupled with tyramide signal amplification (FISH-TSA). The respective cDNA was cloned and sequenced. The labeled probe hybridized with a subcentromeric region of the long arms of homologous chromosomes 3. Results of comparison of the gene localization with previously mapped X. laevis paralogs strongly suggest a common evolutionary origin of chromosomes 3 and 8 in X. laevis and chromosome 3 in X. tropicalis. This is the first time that a single copy gene has been visualized on X. tropicalis chromosomes. The FISH-TSA method gives a strong signal with a 1-kb labeled probe.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::cba05d607721bc69ed98fa7f70bf8bb0 https://pubmed.ncbi.nlm.nih.gov/17268187 Zobrazit plný text záznamu