Simplified Intestinal Microbiota to Study Microbe-Diet-Host Interactions in a Mouse Model

Autor: Kovatcheva-Datchary, Petia, Shoaie, Saeed, Lee, Sunjae, Wahlström, Annika, Nookaew, Intawat, Hallen, Anna, Perkins, Rosie, Nielsen, Jens, Bäckhed, Fredrik
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Cell Reports, Vol 26, Iss 13, Pp 3772-3783.e6 (2019)
Cell Reports
Kovatcheva-Datchary, P, Shoaie, S, Lee, S, Wahlstrom, A, Nookaew, I, Hallen, A, Perkins, R, Nielsen, J & Bäckhed, F 2019, ' Simplified Intestinal Microbiota to Study Microbe-Diet-Host Interactions in a Mouse Model ', Cell Reports, vol. 26, no. 13, pp. 3772-3783.e6 . https://doi.org/10.1016/j.celrep.2019.02.090
Kovatcheva-Datchary, P, Shoaie, S, Lee, S, Wahlström, A, Nookaew, I, Hallen, A, Perkins, R, Nielsen, J & Bäckhed, F 2019, ' Simplified Intestinal Microbiota to Study Microbe-Diet-Host Interactions in a Mouse Model ', Cell Reports, vol. 26, no. 13, pp. 3772-3783 . https://doi.org/10.1016/j.celrep.2019.02.090
ISSN: 2211-1247
Popis: Summary The gut microbiota can modulate human metabolism through interactions with macronutrients. However, microbiota-diet-host interactions are difficult to study because bacteria interact in complex food webs in concert with the host, and many of the bacteria are not yet characterized. To reduce the complexity, we colonize mice with a simplified intestinal microbiota (SIM) composed of ten sequenced strains isolated from the human gut with complementing pathways to metabolize dietary fibers. We feed the SIM mice one of three diets (chow [fiber rich], high-fat/high-sucrose, or zero-fat/high-sucrose diets [both low in fiber]) and investigate (1) how dietary fiber, saturated fat, and sucrose affect the abundance and transcriptome of the SIM community, (2) the effect of microbe-diet interactions on circulating metabolites, and (3) how microbiota-diet interactions affect host metabolism. Our SIM model can be used in future studies to help clarify how microbiota-diet interactions contribute to metabolic diseases.
Graphical Abstract
Highlights • Mice are colonized with ten bacterial strains to create a simple human microbiota model • Dietary changes alter colonization patterns of the simplified intestinal microbiota (SIM) • SIM-diet interactions affect some circulating metabolites in the host • The SIM affects host metabolism in a diet-specific manner
Kovatcheva-Datchary et al. develop a mouse model colonized with a simplified intestinal microbiota (SIM) to investigate the microbe-microbe and host-microbe interactions in the mammalian gut, focusing on host metabolism. They combine dietary interventions and different omics approaches to show the potential of the SIM model to study the microbe-diet-host interplay.
Databáze: OpenAIRE
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