Electroseparation of Slaughterhouse By-Product: Antimicrobial Peptide Enrichment by pH Modification

Autor: Rémi Przybylski, Laurent Bazinet, Loubna Firdaous, Mostafa Kouach, Jean-François Goossens, Pascal Dhulster, Naïma Nedjar-Arroume
Jazyk: angličtina
Předmět:
Zdroj: Membranes
Volume 10
Issue 5
Membranes, Vol 10, Iss 90, p 90 (2020)
ISSN: 2077-0375
DOI: 10.3390/membranes10050090
Popis: The fractionation of bioactive peptides from hydrolysate is a main challenge to produce efficient alternative for synthetic additives. In this work, electrodialysis with ultrafiltration membrane (EDUF) was proposed to increase the purity of one antimicrobial peptide from slaughterhouse by-product hydrolysate. This targeted-peptide, &alpha
137&ndash
141 (653 Da, TSKYR), inhibits a large spectrum of microbial growths and delays meat rancidity
therefore, if concentrated, it could be used as food antimicrobial. In this context, three pH values were investigated during EDUF treatment to increase the &alpha
141 purity: 4.7, 6.5, and 9. pH 9 showed the highest purity increase&mdash
75-fold compared to the initial hydrolysate. Although the whole hydrolysate contains more than 100 peptides, only six peptides were recovered at a significant concentration. In this fraction, the &alpha
141 peptide represented more than 50% of the recovered total peptide concentration. The EDUF &alpha
141-enriched fraction obtained in this optimized condition would be a promising natural preservative to substitute synthetic additives used to protect food.
Databáze: OpenAIRE
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