Bariatric Surgery Induces Disruption in Inflammatory Signaling Pathways Mediated by Immune Cells in Adipose Tissue: A RNA-Seq Study
| Autor: | Poitou, Christine, Perret, Claire, Mathieu, François, Truong, Vinh, Blum, Yuna, Durand, Hervé, Alili, Rohia, Chelghoum, Nadjim, Pelloux, Véronique, Aron-Wisnewsky, Judith, Torcivia, Adriana, Bouillot, Jean-Luc, Parks, Brian W., Ninio, Ewa, Clément, Karine, Tiret, Laurence |
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| Přispěvatelé: | Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases (ICAN), Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), University of California [Los Angeles] (UCLA), University of California (UC), Plateforme Post-génomique de la Pitié-Salpêtrière (P3S), UMS omique (OMIQUE), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Department of general, dugestive and metabolic surgery, Hôpital Ambroise Paré [AP-HP], Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), University of California, Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Inflammation Gene Expression Profiling lcsh:R Bariatric Surgery Computational Biology lcsh:Medicine Middle Aged Disease Models Animal Mice Adipose Tissue Gene Expression Regulation Animals Cluster Analysis Humans Female Gene Regulatory Networks lcsh:Q Interferons Obesity lcsh:Science [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology Research Article Signal Transduction |
| Zdroj: | PLoS ONE PLoS ONE, 2015, 10 (5), pp.e0125718. ⟨10.1371/journal.pone.0125718⟩ PLoS ONE, Vol 10, Iss 5, p e0125718 (2015) PLoS ONE, Public Library of Science, 2015, 10 (5), pp.e0125718. ⟨10.1371/journal.pone.0125718⟩ |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0125718⟩ |
| Popis: | International audience; BackgroundBariatric surgery is associated to improvements in obesity-associated comorbidities thought to be mediated by a decrease of adipose inflammation. However, the molecular mechanisms behind these beneficial effects are poorly understood.Methodology/Principal FindingsWe analyzed RNA-seq expression profiles in adipose tissue from 22 obese women before and 3 months after surgery. Of 15,972 detected genes, 1214 were differentially expressed after surgery at a 5% false discovery rate. Upregulated genes were mostly involved in the basal cellular machinery. Downregulated genes were enriched in metabolic functions of adipose tissue. At baseline, 26 modules of coexpressed genes were identified. The four most stable modules reflected the innate and adaptive immune responses of adipose tissue. A first module reflecting a non-specific signature of innate immune cells, mainly macrophages, was highly conserved after surgery with the exception of DUSP2 and CD300C. A second module reflected the adaptive immune response elicited by T lymphocytes; after surgery, a disconnection was observed between genes involved in T-cell signaling and mediators of the signal transduction such as CXCR1, CXCR2, GPR97, CCR7 and IL7R. A third module reflected neutrophil-mediated inflammation; after surgery, several genes were dissociated from the module, including S100A8, S100A12, CD300E, VNN2, TUBB1 and FAM65B. We also identified a dense network of 19 genes involved in the interferon-signaling pathway which was strongly preserved after surgery, with the exception of DDX60, an antiviral factor involved in RIG-I-mediated interferon signaling. A similar loss of connection was observed in lean mice compared to their obese counterparts.Conclusions/SignificanceThese results suggest that improvements of the inflammatory state following surgery might be explained by a disruption of immuno-inflammatory cascades involving a few crucial molecules which could serve as potential therapeutic targets. |
| Databáze: | OpenAIRE |
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