ATAD2 controls chromatin-bound HIRA turnover
| Autor: | Wang, Tao, Perazza, Daniel, Boussouar, Fayçal, Cattaneo, Matteo, Bougdour, Alexandre, Chuffart, Florent, Barral, Sophie, Vargas, Alexandra, Liakopoulou, Ariadni, Puthier, Denis, Bargier, Lisa, Morozumi, Yuichi, Jamshidikia, Mahya, Garcia-Saez, Isabel, Petosa, Carlo, Rousseaux, Sophie, Verdel, A, Khochbin, Saadi |
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| Přispěvatelé: | Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Theories and Approaches of Genomic Complexity (TAGC), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Transscriptomics and Genomics of Marseille-Luminy (TGML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Nara Institute of Science and Technology, Groupe Épigénétique et voies moléculaires / Epigenetics and Molecular Pathways Group (IBS-EPIGEN), Institut de biologie structurale (IBS - UMR 5075), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Khochbin, Saadi |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Genotype
Cell Cycle Proteins [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Transfection Gene Knockout Techniques Mice Schizosaccharomyces Animals Humans Histone Chaperones Research Articles Cell Proliferation [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Mouse Embryonic Stem Cells Hep G2 Cells Nucleosomes DNA-Binding Proteins ATPases Associated with Diverse Cellular Activities Schizosaccharomyces pombe Proteins Microorganisms Genetically-Modified Gene Deletion Research Article HeLa Cells Signal Transduction Transcription Factors |
| Zdroj: | Life Science Alliance Life Science Alliance, 2021, ⟨10.26508/lsa.202101151⟩ Life Science Alliance, Life Science Alliance LLC, 2021, ⟨10.26508/lsa.202101151⟩ |
| ISSN: | 2575-1077 |
| DOI: | 10.26508/lsa.202101151⟩ |
| Popis: | Parallel study of multiple model systems points to the evolutionary conserved protein ATAD2, as a critical histone chaperone auxiliary factor. Taking advantage of the evolutionary conserved nature of ATAD2, we report here a series of parallel functional studies in human, mouse, and Schizosaccharomyces pombe to investigate ATAD2’s conserved functions. In S. pombe, the deletion of ATAD2 ortholog, abo1, leads to a dramatic decrease in cell growth, with the appearance of suppressor clones recovering normal growth. The identification of the corresponding suppressor mutations revealed a strong genetic interaction between Abo1 and the histone chaperone HIRA. In human cancer cell lines and in mouse embryonic stem cells, we observed that the KO of ATAD2 leads to an accumulation of HIRA. A ChIP-seq mapping of nucleosome-bound HIRA and FACT in Atad2 KO mouse ES cells demonstrated that both chaperones are trapped on nucleosomes at the transcription start sites of active genes, resulting in the abnormal presence of a chaperone-bound nucleosome on the TSS-associated nucleosome-free regions. Overall, these data highlight an important layer of regulation of chromatin dynamics ensuring the turnover of histone-bound chaperones. |
| Databáze: | OpenAIRE |
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