| Autor: |
Ivanov IuD, Pa, Frantsuzov, To, Pleshakova, Vs, Ziborov, Sk, Svetlov, Nv, Krokhin, Va, Konev, Ob, Kovalev, Vf, Uchaĭkin, On, Iastrebova, Peter Sveshnikov, Ai, Archakov |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Europe PubMed Central |
| ISSN: |
2310-6972 |
| Popis: |
The aim of the study was to demonstrate the possibility of detection of serological markers, containing the hepatitis B surface antigen (HBsAg) and hepatitis C virus core-antigen (HCVcoreAg) in human serum, by use of a new atomic force microscopy (AFM)-based nanotechnological approach. In this study, the immobilization on AFM-chip of antibodies against the hepatitis B virus surface antigen (anti-HBsAg) as well as the antibodies against the hepatitis C virus core antigen (anti-HCVcoreAg) was performed. It was shown that such approach enables to detect: HBsAg, HCVcoreAg and the viral fragments containing these antigens in the serum. Comparative analysis of detection of HBsAg- and HCVcoreAg-containing particles by use of the AFM method vs. traditional methods (ELISA, PCR) has demonstrated the 75% coincidence of results between the AFM and the latter two methods. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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