Survival and growth of Stenotrophomonas maltophilia in free-living amoebae (FLA) and bacterial virulence properties

Autor: Denet, Elodie, Vasselon, Valentin, Burdin, Béatrice, Nazaret, Sylvie, Favre-Bonté, Sabine
Přispěvatelé: Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Centre Alpin de Recherche sur les Réseaux Trophiques et Ecosystèmes Limniques (CARRTEL), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Centre National de la Recherche Scientifique, FR BioEnviS, Ministere de l'Education Nationale, de l'Enseignement Superieur et de la Recherche
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Confocal Microscopy
Physiology
Dictyosteliomycota
Stenotrophomonas maltophilia
[SDV]Life Sciences [q-bio]
lcsh:Medicine
Acanthamoeba
Pathology and Laboratory Medicine
stenotrophomonas
Medicine and Health Sciences
Dictyostelium
Free-Living Amoebic Infection
Amoebas
lcsh:Science
Amoeba
Protozoans
[SDV.EE]Life Sciences [q-bio]/Ecology
environment
Microscopy
Virulence
Dictyostelium Discoideum
Eukaryota
Light Microscopy
Protists
Bacterial Pathogens
Experimental Organism Systems
Slime Molds
Medical Microbiology
[SDE]Environmental Sciences
Pathogens
Research Article
pompe d'efflux
Lysis (Medicine)
Imaging Techniques
macromolecular substances
Research and Analysis Methods
Real-Time Polymerase Chain Reaction
Microbiology
Model Organisms
Tissue Repair
Fluorescence Imaging
parasitic diseases
Parasitic Diseases
Microbial Pathogens
virulence bactérienne
Protozoan Models
lcsh:R
Organisms
Biology and Life Sciences
biochemical phenomena
metabolism
and nutrition
Parasitic Protozoans
lcsh:Q
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Physiological Processes
Zdroj: PLoS ONE
PLoS ONE, Public Library of Science, 2018, ⟨10.1371/journal.pone.0192308⟩
Plos One 2 (13), . (2018)
PLoS ONE, Public Library of Science, 2018, 13 (2), ⟨10.1371/journal.pone.0192308⟩
PLoS ONE, Vol 13, Iss 2, p e0192308 (2018)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0192308⟩
Popis: International audience; Stenotrophomonas maltophilia is found ubiquitously in the environment and is an important emerging nosocomial pathogen. S. maltophilia has been recently described as an Amoe-bae-Resistant Bacteria (ARB) that exists as part of the microbiome of various free-living amoebae (FLA) from waters. Co-culture approaches with Vermamoeba vermiformis demonstrated the ability of this bacterium to resist amoebal digestion. In the present study, we assessed the survival and growth of six environmental and one clinical S. maltophilia strains within two amoebal species: Acanthamoeba castellanii and Willaertia magna. We also evaluated bacterial virulence properties using the social amoeba Dictyostelium discoideum. A co-culture approach was carried out over 96 hours and the abundance of S. maltophilia cells was measured using quantitative PCR and culture approach. The presence of bacteria inside the amoeba was confirmed using confocal microscopy. Our results showed that some S. maltophilia strains were able to multiply within both amoebae and exhibited multiplication rates up to 17.5 and 1166 for A. castellanii and W. magna, respectively. In contrast, some strains were unable to multiply in either amoeba. Out of the six environmental S. mal-tophilia strains tested, one was found to be virulent. Surprisingly, this strain previously isolated from a soil amoeba, Micriamoeba, was unable to infect both amoebal species tested. We further performed an assay with a mutant strain of S. maltophilia BurA1 lacking the efflux pump ebyCAB gene and found the mutant to be more virulent and more efficient for intra-amoebal multiplication. Overall, the results obtained strongly indicated that free-living amoe-bae could be an important ecological niche for S. maltophilia.
Databáze: OpenAIRE
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