Chemical techniques employed for the primary structural analysis of proteins and peptides

Autor:	As, Inglis, Ge, Reid, Richard Simpson
Rok vydání:	1995
Předmět:	Molecular Sequence Data Proteins Electrophoresis Gel Two-Dimensional Amino Acid Sequence Amino Acids Peptides Peptide Mapping Sequence Analysis Chromatography High Pressure Liquid Mass Spectrometry
Zdroj:	Europe PubMed Central
ISSN:	1023-294X
Popis:	This chapter summarises modern microchemical approaches to the purification, identification and primary structure analysis of peptides and proteins. Discussion of high-sensitivity purification methods is restricted to two-dimensional polyacrylamide gel electrophoresis (2-DE) and microbore/capillary column reversed-phase high-performance liquid chromatography (RP-HPLC). Associated techniques are discussed, particularly with respect to analysis of the products with current automated amino acid sequencers and mass spectrometers.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::c72e10f2cf2c519c69fe93cf11ddf791 https://pubmed.ncbi.nlm.nih.gov/7579972 Zobrazit plný text záznamu