Outbreak of dengue virus serotype-2 (DENV-2) of Cambodian origin in Manipur, India - Association with meteorological factors
| Autor: | Sankari, T., S.L. Hoti, Singh, T. B., Shanmugavel, J. |
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| Předmět: |
Manipur
Base Sequence Meteorological Concepts DENV-2 Molecular Sequence Data India Computational Biology Sequence Analysis DNA Dengue Virus Real-Time Polymerase Chain Reaction History 21st Century Disease Outbreaks Dengue Species Specificity dengue cases Climatic variables Cluster Analysis Humans RNA Viral Original Article Cambodia Phylogeny dengue outbreak |
| Zdroj: | Scopus-Elsevier The Indian Journal of Medical Research |
| Popis: | Background & objectives: Dengue is a major health problem in many parts of India and its neighbouring countries. Dengue cases have not been reported from Manipur, a northeastern State of India till 2007. But, the sudden outbreak of fever with febrile illness during 2007 and 2008, suspected to be dengue/dengue haemorrhagic fever was investigated to detect the causative agent. Potential impact of climatic variables on dengue transmission has been documented and hence the association between climatic factors, entomological parameters and dengue cases was also analysed. Methods: Forty two and 16 blood samples were collected from patients suspected to have dengue infection in the year 2007 and 2008, respectively. Viral RNA was extracted from serum samples and subjected to multiplex one step RT-PCR assay. Dengue specific amplicons were sequenced and phylogenetic analysis was carried out. Multiyear trend analysis and ‘t’ test were performed for the comparison of different meteorological variables between the years 2000-2004 and 2005-2008. Results: The aetiological agent was found to be DENV-2 and the phylogenetic analysis showed that the isolate was similar to that of Cambodian isolate. There was a significant difference in minimum temperature (P |
| Databáze: | OpenAIRE |
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