Expression of thrombopoietin by umbilical vein endothelial cells
| Autor: | El Andaloussi, A., Duchez, P., Reiffers, J., Ripoche, J., Christophe Grosset |
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| Přispěvatelé: | Grosset, Christophe, Laboratoire de cytogénétique [Casablanca, Maroc], Institut Pasteur du Maroc, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Laboratoire de Greffe de Moelle (LGM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Département de pharmacochimie moléculaire (DPM), Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), The comité mixte inter-universitaire franco-marocain is acknowledged for support (grant # 234/SVS/00)., Martine Renard is acknowledged for the gift of endothelial cells. F. Wendling and W. Vainchenker are acknowledged for the gift of plasmid pME18S containing the full length TPO cDNA, BaF3 and BaF3/MPLR cells., Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF) |
| Předmět: |
MESH: DNA Primers
Umbilical Veins endocrine system MESH: Endothelium Vascular/cytology Blotting Western MESH: Thrombopoietin/metabolism MESH: Base Sequence fluids and secretions MESH: Umbilical Veins/cytology MESH: Thrombopoietin/genetics [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Humans MESH: Blotting Northern MESH: Blotting Western [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology RNA Messenger Cells Cultured DNA Primers MESH: Umbilical Veins/metabolism MESH: Endothelium Vascular/metabolism MESH: Humans Base Sequence MESH: Culture Media Conditioned food and beverages hemic and immune systems Blotting Northern Thrombopoietin Culture Media Conditioned embryonic structures Endothelium Vascular MESH: RNA Messenger/genetics MESH: Cells Cultured |
| Zdroj: | ResearcherID European Cytokine Network European Cytokine Network, 2001, 12 (2), pp.268-73 European Cytokine Network, John Libbey Eurotext, 2001, 12 (2), pp.268-73 |
| ISSN: | 1148-5493 1952-4005 |
| Popis: | International audience; Current data suggest that the primary source of thrombopoietin (TPO) is the liver. Extra-hepatic sites for TPO production have been demonstrated essentially through the study of the expression of TPO mRNA. In this work, we report that TPO is expressed at low levels by endothelial cells (EC) derived from the umbilical vein (HUVEC). Both TPO mRNA and the protein are expressed and the protein is functional as assessed by biological assay. Expression of TPO by HUVEC may be useful to study the regulation of the production of this cytokine and to understand the apparent specific interactions between mature megakaryocytes and EC in the bone marrow. |
| Databáze: | OpenAIRE |
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