How to assess and study cell death in Dictyostelium discoideum
| Autor: | Kosta, Artemis, Laporte, Catherine, Lam, David, Tresse, Emilie, Luciani, Marie-Françoise, Golstein, Pierre |
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| Přispěvatelé: | Centre d'Immunologie de Marseille - Luminy ( CIML ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Aix Marseille Université ( AMU ) -Centre National de la Recherche Scientifique ( CNRS ), Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
MESH: Cell Death
Cell Death MESH : Flow Cytometry MESH: Dictyostelium MESH: Flow Cytometry Flow Cytometry MESH : Autophagy MESH : Dictyostelium Colony-Forming Units Assay MESH : Cell Death Autophagy MESH: Autophagy MESH: Colony-Forming Units Assay [SDV.IMM]Life Sciences [q-bio]/Immunology Animals [ SDV.IMM ] Life Sciences [q-bio]/Immunology MESH: Animals Dictyostelium MESH : Animals MESH : Colony-Forming Units Assay |
| Zdroj: | Methods in Molecular Biology (Clifton then Totova) Methods in Molecular Biology (Clifton then Totova), Humana Press (Springer Imprint), 2006, 346, pp.535-50. 〈10.1385/1-59745-144-4:535〉 Methods in Molecular Biology Methods in Molecular Biology, Humana Press/Springer Imprint, 2006, 346, pp.535-50. ⟨10.1385/1-59745-144-4:535⟩ Methods in Molecular Biology, 2006, 346, pp.535-50. ⟨10.1385/1-59745-144-4:535⟩ |
| ISSN: | 1064-3745 1940-6029 |
| DOI: | 10.1385/1-59745-144-4:535〉 |
| Popis: | In this chapter, we describe how to conveniently demonstrate, assess, and study cell death in Dictyostelium through simple cell culture, clonogenic tests, and photonic (with the help of staining techniques) and electronic microscopy. Cell death can be convniently generated using minor modifications of the monolayer technique of Rob Kay et al., and either wild-type HMX44A Dictyostelium cells or the corresponding atg1- autophagy gene mutant cells. Methods to follow cell death qualitatively and quantitatively facilitate detailed studies of vacuolar death in wild-type cells and of nonvacuolar, "condensed" death in atg1- mutant cells. |
| Databáze: | OpenAIRE |
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