Naturally occurring polymorphisms in the virulence regulator Rsp modulate Staphylococcus aureus survival in blood and antibiotic susceptibility
| Autor: | Krishna, A, Holden, M, Peacock, S, Edwards, A, Wigneshweraraj, S |
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| Přispěvatelé: | University of St Andrews. Infection and Global Health Division, University of St Andrews. Biomedical Sciences Research Complex, University of St Andrews. Infection Group, University of St Andrews. School of Medicine, Biotechnology and Biological Sciences Research Council (BBSRC), Wellcome Trust, The Royal Society, Medical Research Council (MRC) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
EXPRESSION
BACTEREMIA Staphylococcus aureus bacteraemia Blood Bactericidal Activity Virulence Factors Short Communication PATHOPHYSIOLOGY education NDAS VANCOMYCIN RESISTANCE Microbiology NASAL CARRIAGE BINDING MD Multidisciplinary Drug Resistance Bacterial Genes Regulator EPIDEMIOLOGY Humans AGR DYSFUNCTION health care economics and organizations Science & Technology Rsp Microbial Viability Polymorphism Genetic QR Microbiology Staphylococcal Infections EVOLUTIONARY DYNAMICS mutations Virulence regulator Staphylococcus aureas QR Anti-Bacterial Agents Bacteraemia virulence regulator Life Sciences & Biomedicine SYSTEM Mutations Regulation |
| Zdroj: | Microbiology |
| ISSN: | 1465-2080 1350-0872 |
| Popis: | This work was funded by the Imperial College President’s Scholarship awarded to A. K; M. T. G.H acknowledges funding from Chief Scientists Office (Grant Number SIRN10); S. J. P thanks the UKCRC Translational Infection Research Initiative and the Medical Research Council (Grant Number G1000803) for funding to cover the original isolate collection; A. M. E acknowledges funding from the Royal Society, Department of Medicine (Imperial College), and from the Imperial NIHR Biomedical Research Centre, Imperial College London; S.W is a recipient of a Wellcome Trust Investigator Award. Nasal colonization by the pathogen Staphylococcus aureus is a risk factor for subsequent infection. Loss of function mutations in the gene encoding the virulence regulator Rsp are associated with the transition of S. aureus from a colonizing isolate to one that causes bacteraemia. Here, we report the identification of several novel activity-altering mutations in rsp detected in clinical isolates, including for the first time, mutations that enhance agr operon activity. We assessed how these mutations affected infection-relevant phenotypes and found loss and enhancement of function mutations to have contrasting effects on S. aureus survival in blood and antibiotic susceptibility. These findings add to the growing body of evidence that suggests S. aureus 'trades off' virulence for the acquisition of traits that benefit survival in the host, and indicates that infection severity and treatment options can be significantly affected by mutations in the virulence regulator rsp. Publisher PDF |
| Databáze: | OpenAIRE |
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