Gliding associated proteins play essential roles during the formation of the inner membrane complex of toxoplasma gondii
| Autor: | Harding, Clare R., Egarter, Saskia, Gow, Matthew, Jiménez-Ruiz, Elena, Ferguson, David J.P., Meissner, Markus |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Life Cycles
QH301-705.5 Recombinant Fusion Proteins Parasitic Life Cycles Cell Membranes Protozoan Proteins Biosynthesis Biochemistry Parasitic Cell Cycles Parasite Replication Cell Line Toxoplasma Gondii Gene Knockout Techniques Microscopy Electron Transmission Reproduction Asexual Humans Protein Isoforms Biology (General) Protozoans Microscopy Video Organelle Biogenesis Organisms Genetically Modified Cell Membrane Cytoplasmic Vesicles Organisms Membrane Proteins Biology and Life Sciences Cell Biology RC581-607 Recombinant Proteins Parasitic Protozoans Luminescent Proteins Protein Transport Organelle Size Vacuoles Parasitology Immunologic diseases. Allergy Cellular Structures and Organelles Toxoplasma Biomarkers Research Article Developmental Biology |
| Zdroj: | PLoS Pathogens PLoS Pathogens, Vol 12, Iss 2, p e1005403 (2016) |
| ISSN: | 1553-7366 |
| Popis: | The inner membrane complex (IMC) of apicomplexan parasites is a specialised structure localised beneath the parasite’s plasma membrane, and is important for parasite stability and intracellular replication. Furthermore, it serves as an anchor for the myosin A motor complex, termed the glideosome. While the role of this protein complex in parasite motility and host cell invasion has been well described, additional roles during the asexual life cycle are unknown. Here, we demonstrate that core elements of the glideosome, the gliding associated proteins GAP40 and GAP50 as well as members of the GAPM family, have critical roles in the biogenesis of the IMC during intracellular replication. Deletion or disruption of these genes resulted in the rapid collapse of developing parasites after initiation of the cell cycle and led to redistribution of other glideosome components. Author Summary Toxoplasma gondii is an important parasite of humans and animals that must actively invade host cells in order to replicate. Beneath the surface of the parasite lies the inner membrane complex (IMC) which is important in maintaining the stability of the parasite, as well as acting as a base for a protein complex known as the glideosome. This assembly of proteins has an important role in allowing the parasite to invade host cells. Here, we examined the function of proteins known to be part of the glideosome, GAP40, GAP50 and five proteins of the GAPM family. We found that in the absence of GAP40 or GAP50, the parasite is able to start replication but is unable to complete it, suggesting that these proteins have a structural role in maintaining the stability of the developing IMC during replication. We also saw that disruption of some members of the GAPM protein family led to a loss of parasite structure. Our study demonstrates that some components of the glideosome have multiple roles in T. gondii biology and gives us new insights into how cells are constructed during parasite replication. |
| Databáze: | OpenAIRE |
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