Quantitative Assessment of Ciliary Ultrastructure with the Use of Automatic Analysis: PCD Quant

Autor: Felšöová, Andrea, Sloboda, Tibor, Hudec, Lukáš, Koblížek, Miroslav, Pohunek, Petr, Martinů, Vendula, Varényiová, Žofia, Kadlecová, Simona, Uhlík, Jiří
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Diagnostics
Volume 11
Issue 8
Diagnostics, Vol 11, Iss 1363, p 1363 (2021)
ISSN: 2075-4418
DOI: 10.3390/diagnostics11081363
Popis: The ciliary ultrastructure can be damaged in various situations. Such changes include primary defects found in primary ciliary dyskinesia (PCD) and secondary defects developing in secondary ciliary dyskinesia (SCD). PCD is a genetic disease resulting from impaired ciliary motility causing chronic disease of the respiratory tract. SCD is an acquired condition that can be caused, for example, by respiratory infection or exposure to tobacco smoke. The diagnosis of these diseases is a complex process with many diagnostic methods, including the evaluation of ciliary ultrastructure using transmission electron microscopy (the golden standard of examination). Our goal was to create a program capable of automatic quantitative analysis of the ciliary ultrastructure, determining the ratio of primary and secondary defects, as well as analysis of the mutual orientation of cilia in the ciliary border. PCD Quant, a program developed for the automatic quantitative analysis of cilia, cannot yet be used as a stand-alone method for evaluation and provides limited assistance in classifying primary and secondary defect classes and evaluating central pair angle deviations. Nevertheless, we see great potential for the future in automatic analysis of the ciliary ultrastructure.
Databáze: OpenAIRE
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