Peripheral Opioid Receptor Blockade Enhances Epithelial Damage in Piroxicam-Accelerated Colitis in IL-10-Deficient Mice
| Autor: | Mas-Orea, Xavier, Sebert, Morgane, Benamar, Mehdi, Petitfils, Camille, Blanpied, Catherine, Saoudi, Abdelhadi, Deraison, Céline, Barreau, Frederick, Cenac, Nicolas, Dietrich, Gilles |
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| Přispěvatelé: | Institut de Recherche en Santé Digestive (IRSD ), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Toulousain des Maladies Infectieuses et Inflammatoires (Infinity), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), association Francois Aupetit, AFA2019/Association Francois Aupetit, SEGUIN, Nathalie |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
CD4-Positive T-Lymphocytes
MESH: Colitis / chemically induced [SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] colitis Narcotic Antagonists MESH: Animals Deraison MESH: Anti-Inflammatory Agents Non-Steroidal / pharmacology Anti-Inflammatory Agents MESH: Epithelial Cells / drug effects [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Severity of Illness Index M Blanpied Mice MESH: Inflammation / metabolism A Biology (General) Intestinal Mucosa MESH: Apoptosis / genetics Mice Knockout [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology Naloxone scolitis Anti-Inflammatory Agents Non-Steroidal Saoudi MESH: CD4-Positive T-Lymphocytes / drug effects apoptosis MESH: Apoptosis / drug effects Cenac Interleukin-10 Chemistry Mas-Orea Petitfils Sebert Cytokines G. Peripheral Opioid Receptor Blockade Enhances opioids QH301-705.5 [SDV.BC]Life Sciences [q-bio]/Cellular Biology Article Permeability MESH: Colitis / genetics C MESH: Anti-Inflammatory Agents / pharmacology [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] Animals Dietrich X QD1-999 [SDV.BC] Life Sciences [q-bio]/Cellular Biology Benamar Barreau Inflammation intestinal permeability piroxicam MESH: Cytokines / metabolism MESH: Inflammation / genetics opioids Epithelial Cells [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology N MESH: Cytokines / genetics Mice Inbred C57BL Quaternary Ammonium Compounds IL-10-deficient mice F Receptors Opioid opioid MESH: Colitis / metabolism MESH: Colitis / pathology [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, MDPI, 2021, 22 (14), 14 p. ⟨10.3390/ijms22147387⟩ International Journal of Molecular Sciences, 2021, 22 (14), pp.7387. ⟨10.3390/ijms22147387⟩ International Journal of Molecular Sciences, Vol 22, Iss 7387, p 7387 (2021) Volume 22 Issue 14 |
| ISSN: | 1661-6596 1422-0067 |
| DOI: | 10.3390/ijms22147387⟩ |
| Popis: | Mucosal CD4+ T lymphocytes display a potent opioid-mediated analgesic activity in interleukin (IL)-10 knockout mouse model of inflammatory bowel diseases (IBD). Considering that endogenous opioids may also exhibit anti-inflammatory activities in the periphery, we examined the consequences of a peripheral opioid receptor blockade by naloxone-methiodide, a general opioid receptor antagonist unable to cross the blood–brain barrier, on the development of piroxicam-accelerated colitis in IL-10-deficient (IL-10-/-) mice. Here, we show that IL-10-deficient mice treated with piroxicam exhibited significant alterations of the intestinal barrier function, including permeability, inflammation-related bioactive lipid mediators, and mucosal CD4+ T lymphocyte subsets. Opioid receptor antagonization in the periphery had virtually no effect on colitis severity but significantly worsened epithelial cell apoptosis and intestinal permeability. Thus, although the endogenous opioid tone is not sufficient to reduce the severity of colitis significantly, it substantially contributes to the protection of the physical integrity of the epithelial barrier. |
| Databáze: | OpenAIRE |
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