Explorative Screening of Bioactivities Generated by Plant-Based Proteins after In Vitro Static Gastrointestinal Digestion
| Autor: | Dugardin, Camille, Cudennec, Benoit, Tourret, Mélissa, Caron, Juliette, Guérin-Deremaux, Laetitia, Behra-Miellet, Josette, Lefranc-Millot, Catherine, Ravallec, Rozenn |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Protein Hydrolysates
Dipeptidyl Peptidase 4 biological activity lcsh:TX341-641 Peptidyl-Dipeptidase A Article Antioxidants Glucagon-Like Peptide 1 Animals Humans Mass Screening ROS production ACE Plant Proteins Dipeptidyl-Peptidase IV Inhibitors IL-8 Diet Vegetarian Interleukin-8 fungi CCK food and beverages Fabaceae opioid receptor Intestines Whey Proteins DPP-IV inflammation Receptors Opioid Cytokines Digestion Caco-2 Cells Peptides GLP-1 plant-based protein digestion lcsh:Nutrition. Foods and food supply |
| Zdroj: | Nutrients, Vol 12, Iss 3746, p 3746 (2020) Nutrients Volume 12 Issue 12 |
| ISSN: | 2072-6643 |
| Popis: | The gastrointestinal digestion of food proteins can generate peptides with a wide range of biological activities. In this study, we screened various potential bioactivities generated by plant-based proteins. Whey protein as an animal protein reference, five grades of pea protein, two grades of wheat protein, and potato, fava bean, and oat proteins were submitted to in vitro SGID. They were then tested in vitro for several bioactivities including measures on: (1) energy homeostasis through their ability to modulate intestinal hormone secretion, to inhibit DPP-IV activity, and to interact with opioid receptors (2) anti-hypertensive properties through their ability to inhibit ACE activity (3) anti-inflammatory properties in Caco-2 cells (4) antioxidant properties through their ability to inhibit production of reactive oxygen species (ROS). Protein intestinal digestions were able to stimulate intestinal hormone secretion by enteroendocrine cells, to inhibit DPP-IV and ACE activities, to bind opioid receptors, and surprisingly, to decrease production of ROS. Neither pro- nor anti-inflammatory effects have been highlighted and some proteins lost their pro-inflammatory potential after digestion. The best candidates were pea, potato, and fava bean proteins. |
| Databáze: | OpenAIRE |
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