A genome scan for QTL affecting resistance to Haemonchus contortus in sheep
| Autor: | Sallé, Guillaume, Jacquiet, Philippe, Gruner, L., Cortet, Jacques, Sauvé, Christine, Prévot, Françoise, Grisez, Christelle, Bergeaud, Jean-Paul, Schibler, Laurent, Tircazes, Aurelie, François, Dominique, Péry, Christophe, Bouvier, Frédéric, Thouly, Jean-Claude, Brunel, Jean-Claude, Legarra, Andres, Elsen, Jean Michel, Bouix, Jacques, Rupp, Rachel, Moreno, Carole |
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| Přispěvatelé: | Station d'Amélioration Génétique des Animaux (SAGA), Institut National de la Recherche Agronomique (INRA), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Domaine expérimental Bourges-La Sapinière (BOURGES), UR1282, Infectiologie Animale et Santé Publique, UR Infectiologie animale et Santé publique (UR IASP), Institut National de la Recherche Agronomique (INRA)-Université de Tours |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Genetic Markers
Male [SDV.SA]Life Sciences [q-bio]/Agricultural sciences sheep Genotyping Techniques Sheep Diseases Breeding Polymorphism Single Nucleotide Parasite Load resistance linkage disequilibrium quantitative trait loci haemonchus contortus parasitic diseases Animals Parasite Egg Count Crosses Genetic Disease Resistance Oligonucleotide Array Sequence Analysis Hematocrit Female Haemonchus France résistance génétique Haemonchiasis Blood Chemical Analysis Microsatellite Repeats |
| Zdroj: | Journal of Animal Science Journal of Animal Science, American Society of Animal Science, 2012, 90 (13), pp.4690-4705. ⟨10.2527/jas.2012-5121⟩ Journal of Animal Science 13 (90), 4690-4705. (2012) |
| ISSN: | 0021-8812 1525-3163 |
| DOI: | 10.2527/jas.2012-5121⟩ |
| Popis: | Gastrointestinal nematodes are one of the main health issues in sheep breeding. To identify loci affecting the resistance to Haemonchus contortus, a genome scan was carried out using 1,275 Romane × Martinik Black Belly backcross lambs. The entire population was challenged with Haemonchus contortus in 2 consecutive experimental infections, and fecal egg counts (FEC) and packed cell volumes were measured. A subgroup of 332 lambs with extreme FEC was necropsied to determine the total worm burden, length of female worms, sex ratio in the worm population, abomasal pH, and serum and mucosal G immunoglobulins (IgG) responses. Pepsinogen concentration was measured in another subset of 229 lambs. For QTL detection, 160 microsatellite markers were used as well as the Illumina OvineSNP50 BeadChip that provided 42,469 SNP markers after quality control. Linkage, association, and joint linkage and association analyses were performed with the QTLMAP software. Linkage disequilibrium (LD) was estimated within each pure breed, and association analyses were carried out either considering or not the breed origin of the haplotypes. Four QTL regions on sheep chromosomes (OAR)5, 12, 13, and 21 were identified as key players among many other QTL with small to moderate effects. A QTL on OAR21 affecting pepsinogen concentration exactly matched the pepsinogen (PGA5) locus. A 10-Mbp region affecting FEC after the 1st and 2nd infections was found on OAR12. The SNP markers outperformed microsatellites in the linkage analysis. Taking advantage of the LD helped to refine the locations of the QTL mapped on OAR5 and 13. |
| Databáze: | OpenAIRE |
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