Plasma and urine metabolomic analyses in aortic valve stenosis reveal shared and biofluid-specific changes in metabolite levels
Autor: | Al Hageh, Cynthia, Rahy, Ryan, Khazen, Georges, Brial, Francois, Khnayzer, Rony S., Gauguier, Dominique, Zalloua, Pierre A. |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Male
Physiology Science Urine Urinalysis Research and Analysis Methods Biochemistry Gas Chromatography-Mass Spectrometry Mass Spectrometry Blood Plasma Analytical Chemistry Plasma Spectrum Analysis Techniques Metabolites Medicine and Health Sciences Metabolomics Humans Chromatographic Techniques Fatty Acids Biology and Life Sciences Aortic Valve Stenosis Middle Aged Lipids Body Fluids Chemistry Metabolism Blood Echocardiography Aortic Valve Case-Control Studies Heart Valve Prosthesis Physical Sciences Multivariate Analysis Regression Analysis Medicine Female Metabolic Pathways Anatomy Biomarkers Research Article |
Zdroj: | PLoS ONE, Vol 15, Iss 11, p e0242019 (2020) PLoS ONE |
ISSN: | 1932-6203 |
Popis: | Aortic valve stenosis (AVS) is a prevalent condition among the elderly population that eventually requires aortic valve replacement. The lack of reliable biomarkers for AVS poses a challenge for its early diagnosis and the application of preventive measures. Untargeted gas chromatography mass spectrometry (GC-MS) metabolomics was applied in 46 AVS cases and 46 controls to identify plasma and urine metabolites underlying AVS risk. Multivariate data analyses were performed on pre-processed data (e.g. spectral peak alignment), in order to detect changes in metabolite levels in AVS patients and to evaluate their performance in group separation and sensitivity of AVS prediction, followed by regression analyses to test for their association with AVS. Through untargeted analysis of 190 urine and 130 plasma features that could be detected and quantified in the GC-MS spectra, we identified contrasting levels of 22 urine and 21 plasma features between AVS patients and control subjects. Following metabolite assignment, we observed significant changes in the concentration of known metabolites in urine (n = 14) and plasma (n = 15) that distinguish the metabolomic profiles of AVS patients from healthy controls. Associations with AVS were replicated in both plasma and urine for about half of these metabolites. Among these, 2-Oxovaleric acid, elaidic acid, myristic acid, palmitic acid, estrone, myo-inositol showed contrasting trends of regulation in the two biofluids. Only trans-Aconitic acid and 2,4-Di-tert-butylphenol showed consistent patterns of regulation in both plasma and urine. These results illustrate the power of metabolomics in identifying potential disease-associated biomarkers and provide a foundation for further studies towards early diagnostic applications in severe heart conditions that may prevent surgery in the elderly. |
Databáze: | OpenAIRE |
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