Changes in human mucosal gamma delta T cell repertoire and function associated with the disease process in inflammatory bowel disease
| Autor: | Ld, Mcvay, Li B, Biancaniello R, Ma, Creighton, Bachwich D, Lichtenstein G, Jl, Rombeau, Simon Carding |
|---|---|
| Předmět: |
Adult
Aged 80 and over Male Colon Biopsy T-Lymphocytes Receptors Antigen T-Cell gamma-delta Fibroblasts Middle Aged Inflammatory Bowel Diseases Polymerase Chain Reaction digestive system diseases Interferon-gamma Cell Adhesion Humans Female Lymphocyte Count Immunity Mucosal In Situ Hybridization Aged Research Article |
| Zdroj: | Europe PubMed Central |
| Popis: | BACKGROUND: Although gamma delta T cells are a major component of the human intestinal mucosa, it is not clear what role they play in mucosal immunity or if they are involved in the disease process of inflammatory bowel disease (IBD). MATERIALS AND METHODS: Flow cytometry and reverse transcriptase-polymerase chain reaction (RT-PCR) assays were used to identify quantitative and qualitative changes in the repertoire of gamma delta T cells present in surgical and/or biopsy samples or normal and inflamed colon from individual patients with ulcerative colitis (UC) or Crohn's disease (CD). Cytokine production and the ability to adhere to and interact with colonic fibroblasts were used to compare the functional properties of gamma delta T cells isolated from the normal and diseased colonic mucosa. RESULTS: Increased numbers of gamma delta T cells localized in areas of inflammation and tissue injury were found in the majority of patients, irrespective of the type of IBD present. This expansion was attributable to an increase in V delta 1+ cells expressing a V delta 1-(D delta 3)-J delta 1-encoded T cell receptor and was seen in patients with severe disease as well as those with newly diagnosed or less severe forms of IBD. Among T cells present in the inflamed mucosa of patients with CD, gamma delta T cells, particularly V delta 1+ cells, were a major source of the proinflammatory cytokine interferon-gamma and could interact with colonic fibroblasts. CONCLUSIONS: Our results demonstrate that the chronic inflammatory immune response characteristic of IBD is associated with distinct changes in the number, distribution, composition, and function of mucosal gamma delta T cells. Through the production of cytokines and physical interaction with other cells, gamma delta T cells can perform an immunoregulatory function and contribute to the pathophysiology of IBDs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|