Programmed Death-Ligand 1 (PD-L1) Expression Is Induced by Insulin in Pancreatic Ductal Adenocarcinoma Cells Pointing to Its Role in Immune Checkpoint Control
| Autor: | Heckl, Steffen M., Mau, Franziska, Senftleben, Anke, Daunke, Tina, Beckinger, Silje, Abdullazade, Samir, Schreiber, Stefan, Röcken, Christoph, Sebens, Susanne, Schäfer, Heiner |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Medical Sciences, Vol 9, Iss 48, p 48 (2021) Medical Sciences Volume 9 Issue 3 |
| ISSN: | 2076-3271 |
| Popis: | Simple Summary Type-2 Diabetes is an established risk factor of pancreatic cancer, yet the exact modalities as well as the contribution of hyperinsulinemia are not fully understood. In our study, we explored a possible link between insulin and the immune evasion of pancreatic cancer cells. We could demonstrate that insulin is able to induce expression of Programmed Death-Ligand 1 (PD-L1) representing a key molecule in immune checkpoint control. This effect of insulin greatly depends on insulin receptor-A and/or IGF-receptor-induced ERK signaling and is enhanced via a crosstalk with EGF. Through insulin-induced PD-L1 expression, pancreatic cancer cells suppress proliferation of CD8+ T-cells underscoring the potential of insulin to confer immune evasion through this immune checkpoint regulator. Furthermore, some cases out of a cohort of pancreatic cancer patients reveal coexpression of PD-L1 and the cytoplasmic insulin receptor in the tumor tissue as shown by immunohistochemistry. Abstract Type-2 diabetes (T2DM) is a risk factor for the development of pancreatic ductal adenocarcinoma (PDAC) and is characterized by insulin resistance and hyperinsulinemia. Besides the well-known growth-promoting activity of insulin or the other members of the Insulin/Insulin-like Growth factor (IGF) axis, we here describe an inducing effect of insulin on PD-L1 expression in PDAC cells. Treatment of the PDAC cell lines BxPc3, A818-6, and T3M4 with insulin increased PD-L1 expression in a time- and dose dependent fashion, as shown by Western blot and qPCR analysis. siRNA mediated knock-down showed that the effects of insulin on PD-L1 depend on the insulin and IGF receptors (InsR and IGFR, respectively). In addition, a crosstalk of insulin-induced ERK activation and Epidermal Growth Factor (EGF) triggered PD-L1 expression. This involves different mechanisms in the three cell lines including upregulation of InsR-A expression in A818-6 and modulation of the adaptor protein Gab1 in BxPc3 cells. As a consequence of the insulin-induced PD-L1 expression, PDAC cells suppress the proliferation of activated human CD8+ T-cells in coculture experiments. The suppression of CD8+ cell proliferation by insulin-pretreated PDAC cells was reversed by PD-1 blockade with Pembrolizumab or by PD-L1 siRNA. Furthermore, the clinical relevance of these observations was supported by detecting a coexpression of cytoplasmic InsR (characteristic for its activation) and PD-L1 in tumor tissues from PDAC patients. Our findings provide a novel insight into the protumorigenic role of insulin in PDAC. Recognizing the impact of insulin on PD-L1 expression as part of the immune privilege, strategies to interfere with this mechanism could pave the way towards a more efficient immunotherapy of PDAC. |
| Databáze: | OpenAIRE |
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