Spire-1 contributes to the invadosome and its associated invasive properties
| Autor: | Lagal, Vanessa, Abrivard, Marie, Gonzalez, Virginie, Perazzi, Audrey, Popli, Sonam, Verzeroli, Elodie, Tardieux, Isabelle |
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| Přispěvatelé: | Institut Cochin (UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Centre National de la Recherche Scientifique (CNRS), Work was funded by the French Government Research Agency [grant number MIERPV08023KSA], and the Fondation pour la Recherche Médicale' agency [grant number DEQ20100318279]. V.L. is a senior post-doctoral fellow financed by an EC Marie Curie integration grant [grant number PIRG05-GA2009-249158] and the FRM [grant number DEQ20100318279]., Work was funded by the French Government Research Agency [grant numberMIERPV08023KSA], and the ‘Fondation pour la Recherche Me ́dicale’ agency [grantnumber DEQ20100318279]. V.L. is a senior post-doctoral fellow financed by an ECMarie Curie integration grant [grant number PIRG05-GA-2009-249158] and the FRM[grant number DEQ20100318279]. The Nikon confocal imaging facilities weresponsored by the ‘Domaines d’Intérêts Majeurs’ DIM MALINF’’ [grant numbersR11042KK RPH11042KKA], and FRM [grant number DEQ20100318279]., Institut Cochin (IC UM3 (UMR 8104 / U1016)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Bos, Mireille, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2013 |
| Předmět: |
Gelatin invasion
Recombinant Fusion Proteins Formins Nerve Tissue Proteins macromolecular substances Src-activated 3T3 Mice Structure-Activity Relationship Cell Movement [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Animals Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Gene Silencing Pseudopodia Phosphorylation Endothelial transmigration Actin Cell Line Transformed Microfilament Proteins Nuclear Proteins Spire-1 Invadosome 3T3 Cells Actins rab3A GTP-Binding Protein Extracellular Matrix Protein Structure Tertiary HEK293 Cells src-Family Kinases Multiprotein Complexes Vesicular trafficking Carrier Proteins Protein Binding |
| Zdroj: | Journal of Cell Science Journal of Cell Science, Company of Biologists, 2014, 127 (Pt 2), pp.328-40. 〈10.1242/jcs.130161〉 Journal of Cell Science, Company of Biologists, 2014, pp.328-340. 〈10.1242/jcs.130161〉 Journal of Cell Science, Company of Biologists, 2014, 127 (Pt 2), pp.328-40. ⟨10.1242/jcs.130161⟩ Journal of Cell Science, 2014, 127 (Pt 2), pp.328-40. ⟨10.1242/jcs.130161⟩ Journal of Cell Science, 2014, pp.328-340. ⟨10.1242/jcs.130161⟩ Journal of Cell Science, Company of Biologists, 2014, pp.328-340. ⟨10.1242/jcs.130161⟩ |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.130161〉 |
| Popis: | International audience; Cancer cells have an increased ability to squeeze through extracellular matrix gaps that they create by promoting proteolysis of its components. Major sites of degradation are specialized micro-domains in the plasma membrane collectively named invadosomes where the Arp2/3 complex and formin proteins cooperate to spatio-temporally control actin nucleation and the folding of a dynamic F-actin core. At invadosomes, proper coupling of exo-endocytosis allows polarized delivery of proteases that facilitate degradation of ECM and disruption of the cellular barrier. We investigated the contribution of the actin nucleator Spire-1 to invadosome structure and function, using Src-activated cells and cancer cells. We found that Spire-1 is specifically recruited at invadosomes and is part of a multi-molecular complex containing Src kinase, the formin mDia1 and actin. Spire-1 interacts with the Rab3A GTPase, a key player in the regulation of exocytosis that is present at invadosomes. Finally, over- and under-expression of Spire-1 resulted in cells with an increased or decreased potential for matrix degradation, respectively, therefore suggesting a functional interplay of Spire-1 with both actin nucleation and vesicular trafficking that might impact on cell invasive and metastatic behavior. |
| Databáze: | OpenAIRE |
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