NRF2 is a key regulator of endothelial microRNA expression under proatherogenic stimuli
| Autor: | Linna-Kuosmanen, Suvi, Tomas Bosch, Vanesa, Moreau, Pierre R, Bouvy-Liivrand, Maria, Niskanen, Henri, Kansanen, Emilia, Kivelä, Annukka, Hartikainen, Juha, Hippeläinen, Mikko, Kokki, Hannu, Tavi, Pasi, Levonen, Anna-Liisa, Kaikkonen, Minna U |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
NF-E2-Related Factor 2
Gene Expression Profiling Endothelial Cells Original Articles Atherosclerosis Plaque Atherosclerotic MicroRNAs Oxidative Stress Gene Expression Regulation miR-21-5p • miR-100-5p • NRF2 • Atherosclerosis Databases Genetic Human Umbilical Vein Endothelial Cells Phosphatidylcholines Genetics Humans Gene Regulatory Networks AcademicSubjects/MED00200 Transcriptome Oxidation-Reduction Cells Cultured Cellular Senescence |
| Zdroj: | Cardiovascular Research |
| ISSN: | 1755-3245 0008-6363 |
| Popis: | Aims Oxidized phospholipids and microRNAs (miRNAs) are increasingly recognized to play a role in endothelial dysfunction driving atherosclerosis. NRF2 transcription factor is one of the key mediators of the effects of oxidized phospholipids, but the gene regulatory mechanisms underlying the process remain obscure. Here, we investigated the genome-wide effects of oxidized phospholipids on transcriptional gene regulation in human umbilical vein endothelial cells and aortic endothelial cells with a special focus on miRNAs. Methods and results We integrated data from HiC, ChIP-seq, ATAC-seq, GRO-seq, miRNA-seq, and RNA-seq to provide deeper understanding of the transcriptional mechanisms driven by NRF2 in response to oxidized phospholipids. We demonstrate that presence of NRF2 motif and its binding is more prominent in the vicinity of up-regulated transcripts and transcriptional initiation represents the most likely mechanism of action. We further identified NRF2 as a novel regulator of over 100 endothelial pri-miRNAs. Among these, we characterize two hub miRNAs miR-21-5p and miR-100-5p and demonstrate their opposing roles on mTOR, VEGFA, HIF1A, and MYC expressions. Finally, we provide evidence that the levels of miR-21-5p and miR-100-5p in exosomes are increased upon senescence and exhibit a trend to correlate with the severity of coronary artery disease. Conclusion Altogether, our analysis provides an integrative view into the regulation of transcription and miRNA function that could mediate the proatherogenic effects of oxidized phospholipids in endothelial cells. Graphical Abstract |
| Databáze: | OpenAIRE |
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