| Autor: |
Lynne Hampson, Hampson L, Pinkoski M, Cross M, Cm, Heyworth, Rc, Bleackley, Atkinson E, Tm, Dexter |
| Rok vydání: |
1997 |
| Předmět: |
|
| Zdroj: |
Europe PubMed Central |
| ISSN: |
0006-4971 |
| Popis: |
We have identified a gene that has a high level of mRNA expression in undifferentiated, multipotential hematopoietic cells (FDCP-Mix) and that downregulates both transcript and protein, as these cells are induced to differentiate into mature myeloid cells. Sequence analysis of this gene has identified it as a serine protease inhibitor EB22/3 (serpin 2A). Constitutive expression of serpin 2A in FDCP-Mix cells was associated with an increase in the clonogenic potential of the cells and with a delay in the appearance of fully mature cells in cultures undergoing granulocyte macrophage differentiation when compared with control cells. Serpin 2A was also found to be expressed in bone marrow-derived bipotent granulocyte macrophage progenitor cells (GM-colony forming cell [CFC]), but not in erythrocyte progenitor cells from day 15 fetal liver. Expression of serpin 2A also showed a marked up regulation during the activation of cytotoxic suppressor CD8+ T cells, with a clear lag between the appearance of transcript and detection of protein. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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