Customized frozen embryo transfer after identification of the receptivity window with a transcriptomic approach improves the implantation and live birth rates in patients with repeated implantation failure

Autor: Haouzi, Delphine, Entezami, Frida, Torre, Antoine, Innocenti, Charlène, Antoine, Yannick, Mauries, Charlotte, Vincens, Claire, Bringer-Deutsch, Sophie, Gala, Anna, Ferrieres-HOA, Alice, Ohl, Jeanine, Gonzalez Marti, Beatriz, Brouillet, Sophie, Hamamah, Samir
Přispěvatelé: Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Hôpital Arnaud de Villeneuve [CHRU Montpellier], Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Développement Embryonnaire, Fertilité et Environnement (DEFE), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Hôpital Américain de Paris, Clinique de la Muette, University of Nottingham, UK (UON), Centre Médico-chirurgical Obstétrique (CMCO ), CHU Strasbourg, Biologie du Cancer et de l'Infection (BCI ), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), This work was partially supported by a grant from the Gedeon Richter Pharmaceutical Company (N° FORWARD2018_4), Salvy-Córdoba, Nathalie, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Adult
Time Factors
Pregnancy Rate
MESH: Treatment Failure
Fertilization in Vitro
[SDV.MHEP.GEO]Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics
Win-Test
MESH: Gene Expression Profiling
MESH: Embryo Implantation
MESH: Pregnancy
Pregnancy
Receptivity window
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
MESH: Cryopreservation
Humans
Embryo Implantation
Prospective Studies
Treatment Failure
MESH: Infertility
Cryopreservation
MESH: Pregnancy Rate
MESH: Humans
Gene Expression Profiling
MESH: Transcriptome
MESH: Time Factors
MESH: Adult
MESH: Embryo Transfer
Embryo Transfer
MESH: Prospective Studies
MESH: Fertilization in Vitro
MESH: France
[SDV.MHEP.GEO] Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics
Implantation window
Infertility
[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
MESH: Live Birth
Original Article
Female
France
Transcriptome
Live Birth
MESH: Female
Zdroj: Reproductive Sciences
Reproductive Sciences, 2021, 28 (1), pp.69-78. ⟨10.1007/s43032-020-00252-0⟩
ISSN: 1933-7191
1933-7205
DOI: 10.1007/s43032-020-00252-0⟩
Popis: The aim of this prospective study was to evaluate outcome benefits expected in repeated implantation failure (RIF) patients (n = 217) after customized embryo transfer based upon identification of the receptivity window by transcriptomic approach using the Win-Test. In this test, the expression of 11 endometrial genes known to be predictive of endometrial receptivity is assessed by RT-PCR in biopsies collected during the implantation window (6–9 days after the spontaneous luteinizing hormone surge during natural cycles, 5–9 days after progesterone administration during hormone replacement therapy cycles). Then, patients underwent either customized embryo transfer (cET, n = 157 patients) according to the Win-Test results or embryo transfer according to the classical procedure (control group, n = 60). Pregnancy and live birth rates were compared in the two groups. The Win-Test showed that in 78.5% of women, the receptivity window lasted less than 48 h, although it could be shorter ( 48 h, 12%). This highlighted that only in 20% of patients with RIF the endometrium would have been receptive if the classical embryo transfer protocol was followed. In the other 80% of patients, the receptivity window was delayed by 1–3 days relative to the classical timing. This suggests that implantation failure could be linked to inadequate timing of embryo transfer. In agreement, both implantation (22.7% vs. 7.2%) and live birth rates per patient (31.8% vs. 8.3%) were significantly higher in the cET group than in the control group. cET on the basis of the Win-Test results could be proposed to improve pregnancy and live birth rates. ClinicalTrials.gov ID: NCT04192396; December 5, 2019, retrospectively registered. Electronic supplementary material The online version of this article (10.1007/s43032-020-00252-0) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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