Chorioamnionitis Induces a Specific Signature of Placental ABC Transporters Associated with an Increase of miR-331-5p in the Human Preterm Placenta
| Autor: | Eustaquio do Imperio, Guinever, Bloise, Enrrico, Javam, Mohsen, Lye, Phetcharawan, Constantinof, Andrea, Dunk, Caroline, dos Reisf, Fernando Marcos, James Lye, Stephen, Gibb, William, Ortiga-Carvalho, Tania Maria, Matthews, Stephen Giles |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male Placenta Gestational Age Real-Time Polymerase Chain Reaction Severity of Illness Index lcsh:Physiology lcsh:Biochemistry Young Adult Pregnancy ATP Binding Cassette Transporter Subfamily G Member 2 Humans Preterm delivery lcsh:QD415-436 ATP Binding Cassette Transporter Subfamily B Member 1 P-glycoprotein (P-gp) Original Paper lcsh:QP1-981 Gene Expression Profiling Interleukin-8 Infant Newborn Immunohistochemistry Multidrug Resistance-Associated Protein 2 Neoplasm Proteins Up-Regulation MicroRNAs Chorioamnionitis ABC transporters Breast cancer resistance protein (BCRP) Premature Birth ATP-Binding Cassette Transporters Female |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 45, Iss 2, Pp 591-604 (2018) Cellular Physiology and Biochemistry |
| ISSN: | 1421-9778 1015-8987 |
| Popis: | Background/Aims: The ATP-binding cassette (ABC) transporters mediate drug biodisposition and immunological responses in the placental barrier. In vitro infective challenges alter expression of specific placental ABC transporters. We hypothesized that chorioamnionitis induces a distinct pattern of ABC transporter expression. Methods: Gene expression of 50 ABC transporters was assessed using TaqMan® Human ABC Transporter Array, in preterm human placentas without (PTD; n=6) or with histological chorioamnionitis (PTDC; n=6). Validation was performed using qPCR, immunohistochemistry and Western blot. MicroRNAs known to regulate P-glycoprotein (P-gp) were examined by qPCR. Results: Up-regulation of ABCB9, ABCC2 and ABCF2 mRNA was detected in chorioamnionitis (p |
| Databáze: | OpenAIRE |
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