Humoral Immunity Links Candida albicans Infection and Celiac Disease
| Autor: | Corouge, Marion, Loridant, Séverine, Fradin, Chantal, Salleron, Julia, Damiens, Sébastien, Moragues, Maria Dolores, Souplet, Vianney, Jouault, Thierry, Robert, Raymond, Dubucquoi, Sylvain, Sendid, Boualem, Colombel, Jean Frédéric, Poulain, Daniel |
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| Přispěvatelé: | Service des Maladies de l'Appareil Digestif et de la Nutrition [CHRU Lille], Hôpital Claude Huriez [Lille], CHU Lille-CHU Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Université Lille Nord de France (COMUE), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Lille Inflammation Research International Center - U 995 (LIRIC), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Registre EPIMAD, CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-CHU Amiens-Picardie-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Inflammation: mécanismes et régulation et interactions avec la nutrition et les candidoses, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé, Universidad del Pais Vasco / Euskal Herriko Unibertsitatea [Espagne] (UPV/EHU), Innobioships [Lille], Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), Institut d'Immunologie [CHRU Lille], Pôle de Biologie Pathologie Génétique [CHU Lille], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Service de Parasitologie-Mycologie [CHRU LIlle], Institut de Microbiologie [CHRU Lille], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Pôle de Biologie Pathologie Génétique [CHU Lille], Icahn School of Medicine at Mount Sinai [New York] (MSSM), CHU Lille-CHU Lille-Fédération Hospitalière de France-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), The research leading to these results has received funding from the European Community’s Seventh Framework Programme (FP7-2007-2013) under HEALTH-F2-2010-260338-ALLFUN., This work is dedicated to the memory of Professor José Ponton. The authors wish to acknowledge the contribution of Nadine François for serum bank management, Dr Nicolas de Suray for his dedication and involvement to this work, Drs Nadine Cerf-Bensussan, Renata Polakowska Malika Baldwick and Bana Jabri for reviewing the manuscript and helpful discussions, and Dr Dominique Deplanque for his exchanges with the Ethics Committee of Lille University hospital., Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-CHU Amiens-Picardie, Fédération Hospitalière de France-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Hôpital Claude Huriez [Lille], CHU Lille-CHU Lille |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
BIOCHEMISTRY AND MOLECULAR BIOLOGY MESH: Peptides/immunology lcsh:Medicine tissue transglutaminase MESH: Candida albicans/physiology Gliadin MESH: Candidiasis/microbiology Candida albicans antibodies Enzyme-linked immunoassays lcsh:Science [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology MESH: Aged MESH: Middle Aged MESH: Antibodies Fungal/isolation & purification MESH: Candidiasis/blood MESH: Immunoblotting Candidiasis MESH: Enzyme-Linked Immunosorbent Assay Cross reactivity invasive candidiasis Middle Aged in vivo Serology T-cell epitope MESH: Young Adult AGRICULTURAL AND BIOLOGICAL SCIENCES MESH: Cross Reactions/immunology Electrophoresis Polyacrylamide Gel Female MESH: Candidiasis/complications MESH: Antibodies Fungal/immunology Research Article MESH: Candidiasis/immunology Adult MESH: Biomarkers/blood Adolescent Immunoblotting T cells MESH: Immunity Humoral chronic mucocutaneous candidiasis Enzyme-Linked Immunosorbent Assay Cross Reactions Fluorescence Fungal Proteins Young Adult Humans dendritic cells MESH: Gliadin/immunology MESH: Celiac Disease/blood MESH: Celiac Disease/complications MESH: Celiac Disease/immunology Antibodies Fungal Aged MESH: Adolescent MESH: Fungal Proteins/immunology MESH: Humans MEDICINE MESH: Celiac Disease/microbiology MESH: Fluorescence lcsh:R fungi MESH: Adult sprue MESH: Male Immunity Humoral Celiac Disease Sequence motif analysis gene expression responses lcsh:Q Peptides MESH: Female Gluten Biomarkers MESH: Electrophoresis Polyacrylamide Gel |
| Zdroj: | PLoS ONE Addi. Archivo Digital para la Docencia y la Investigación instname PLoS ONE, Vol 10, Iss 3, p e0121776 (2015) PLoS ONE, 2015, 10 (3), pp.e0121776. ⟨10.1371/journal.pone.0121776⟩ PLoS ONE, Public Library of Science, 2015, 10 (3), pp.e0121776. ⟨10.1371/journal.pone.0121776⟩ |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0121776⟩ |
| Popis: | Objective The protein Hwp1, expressed on the pathogenic phase of Candida albicans, presents sequence analogy with the gluten protein gliadin and is also a substrate for transglutaminase. This had led to the suggestion that C. albicans infection (CI) may be a triggering factor for Celiac disease (CeD) onset. We investigated cross-immune reactivity between CeD and CI. Methods Serum IgG levels against recombinant Hwp1 and serological markers of CeD were measured in 87 CeD patients, 41 CI patients, and 98 healthy controls (HC). IgA and IgG were also measured in 20 individuals from each of these groups using microchips sensitized with 38 peptides designed from the N-terminal of Hwp1. Results CI and CeD patients had higher levels of anti-Hwp1 (p= 0.0005 and p= 0.004) and anti-gliadin (p= 0.002 and p= 0.0009) antibodies than HC but there was no significant difference between CeD and CI patients. CeD and CI patients had higher levels of anti-transglutaminase IgA than HC (p= 0.0001 and p= 0.0039). During CI, the increase in anti-Hwp1 paralleled the increase in anti-gliadin antibodies. Microchip analysis showed that CeD patients were more reactive against some Hwp1 peptides than CI patients, and that some deamidated peptides were more reactive than their native analogs. Binding of IgG from CeD patients to Hwp1 peptides was inhibited by gamma III gliadin peptides. Conclusions Humoral cross-reactivity between Hwp1 and gliadin was observed during CeD and CI. Increased reactivity to Hwp1 deamidated peptide suggests that transglutaminase is involved in this interplay. These results support the hypothesis that CI may trigger CeD onset in genetically-susceptible individuals. This work received the following grant support: Unit U995-2 INSERM-Lille2 University; the European Community's 7th Framework program (FP7-2007-2013) grant agreement No Health F2-2010-260338 (ALLFUN); Digest Science Foundation. |
| Databáze: | OpenAIRE |
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