Distribution of Medically Relevant Antibiotic Resistance Genes and Mobile Genetic Elements in Soils of Temperate Forests and Grasslands Varying in Land Use
| Autor: | Willms, I., Yuan, J., Penone, C., Goldmann, K., Vogt, J., Wubet, T., Schöning, I., Schrumpf, M., Buscot, F., Nacke, H. |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
class 1 integrons
lcsh:QH426-470 580 Plants (Botany) Forests Real-Time Polymerase Chain Reaction Article Integrons Fungal Proteins forest antibiotic resistance genes Bacterial Proteins mowing Soil Microbiology IncP-1 plasmids fertilization grassland horizontal gene transfer land use mobile genetic elements Bacteria Fungi Agriculture Drug Resistance Microbial Sequence Analysis DNA biochemical phenomena metabolism and nutrition lcsh:Genetics Macrolides incp-1 plasmids Environmental Monitoring Plasmids |
| Zdroj: | Genes, Vol 11, Iss 2, p 150 (2020) Genes Willms, Inka M.; Yuan, Jingyue; Penone, Caterina; Goldmann, Kezia; Vogt, Juliane; Wubet, Tesfaye; Schöning, Ingo; Schrumpf, Marion; Buscot, François; Nacke, Heiko (2020). Distribution of Medically Relevant Antibiotic Resistance Genes and Mobile Genetic Elements in Soils of Temperate Forests and Grasslands Varying in Land Use. Genes, 11(2), p. 150. MDPI, Molecular Diversity Preservation International 10.3390/genes11020150 |
| DOI: | 10.3390/genes11020150 |
| Popis: | Antibiotic-resistant pathogens claim the lives of thousands of people each year and are currently considered as one of the most serious threats to public health. Apart from clinical environments, soil ecosystems also represent a major source of antibiotic resistance determinants, which can potentially disseminate across distinct microbial habitats and be acquired by human pathogens via horizontal gene transfer. Therefore, it is of global importance to retrieve comprehensive information on environmental factors, contributing to an accumulation of antibiotic resistance genes and mobile genetic elements in these ecosystems. Here, medically relevant antibiotic resistance genes, class 1 integrons and IncP-1 plasmids were quantified via real time quantitative PCR in soils derived from temperate grasslands and forests, varying in land use over a large spatial scale. The generated dataset allowed an analysis, decoupled from regional influences, and enabled the identification of land use practices and soil characteristics elevating the abundance of antibiotic resistance genes and mobile genetic elements. In grassland soils, the abundance of the macrolide resistance gene mefA as well as the sulfonamide resistance gene sul2 was positively correlated with organic fertilization and the abundance of aac(6′)-lb, conferring resistance to different aminoglycosides, increased with mowing frequency. With respect to forest soils, the beta-lactam resistance gene blaIMP-12 was significantly correlated with fungal diversity which might be due to the fact that different fungal species can produce beta-lactams. Furthermore, except blaIMP-5 and blaIMP-12, the analyzed antibiotic resistance genes as well as IncP-1 plasmids and class-1 integrons were detected less frequently in forest soils than in soils derived from grassland that are commonly in closer proximity to human activities. |
| Databáze: | OpenAIRE |
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