Approaches to study differentiation and repair of human airway epithelial cells
| Autor: | Crespin Sophie, Bacchetta Marc, Huang Song, Dudez Tecla, Wiszniewski Ludovic, Chanson Marc |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Wound Healing
ddc:618 Mucins/analysis/biosynthesis Interleukin-8 Epithelial Cells/cytology/metabolism Cell Culture Techniques Mucins Cell Differentiation Epithelial Cells Cell Communication Respiratory Mucosa respiratory system Immunohistochemistry Models Biological respiratory tract diseases Interleukin-8/analysis/biosynthesis Mice Wound Healing/physiology Research Design Cell Communication/physiology Respiratory Mucosa/cytology/metabolism Cell Differentiation/physiology Animals Humans |
| Zdroj: | Methods in Molecular Biology, Vol. 742 (2011) pp. 173-85 Methods in molecular biology (Clifton N.J.) |
| ISSN: | 1940-6029 1064-3745 |
| Popis: | One of the main functions of the airway mucosa is to maintain a mechanical barrier at the air-surface interface and to protect the respiratory tract from external injuries. Differentiation of human airway epithelial cells (hAECs) to polarized airway mucosa can be reproduced in vitro by culturing the cells on microporous membrane at the air-liquid interface. Here, we describe approaches to study differentiation as well as repair of the hAECs by using a commercially available airway cell culture model called MucilAir™. |
| Databáze: | OpenAIRE |
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