Selective flexible packaging pathways of the segmented genome of influenza A virus
| Autor: | Haralampiev, Ivan, Prisner, Simon, Nitzan, Mor, Schade, Matthias, Jolmes, Fabian, Schreiber, Max, Loidolt-Krüger, Maria, Jongen, Kalle, Chamiolo, Jasmine, Nilson, Niklaas, Winter, Franziska, Friedman, Nir, Seitz, Oliver, Wolff, Thorsten, Herrmann, Andreas |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Science
Fluorescence in situ hybridization Influenza A Virus H3N2 Subtype Virus Assembly viruses Epithelial Cells Models Theoretical Article Evolution Molecular 500 Naturwissenschaften und Mathematik Ribonucleoproteins A549 Cells Influenza A virus Influenza Human Humans RNA Viral lcsh:Q ddc:500 lcsh:Science Influenza virus Viral evolution Lung Viral genetics In Situ Hybridization |
| Zdroj: | Nature Communications Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020) |
| Popis: | The genome of influenza A viruses (IAV) is encoded in eight distinct viral ribonucleoproteins (vRNPs) that consist of negative sense viral RNA (vRNA) covered by the IAV nucleoprotein. Previous studies strongly support a selective packaging model by which vRNP segments are bundling to an octameric complex, which is integrated into budding virions. However, the pathway(s) generating a complete genome bundle is not known. We here use a multiplexed FISH assay to monitor all eight vRNAs in parallel in human lung epithelial cells. Analysis of 3.9 × 105 spots of colocalizing vRNAs provides quantitative insights into segment composition of vRNP complexes and, thus, implications for bundling routes. The complexes rarely contain multiple copies of a specific segment. The data suggest a selective packaging mechanism with limited flexibility by which vRNPs assemble into a complete IAV genome. We surmise that this flexibility forms an essential basis for the development of reassortant viruses with pandemic potential. The mechanism underlying packaging of the 8 segments of the influenza virus genome into virions is not well understood. Here, the authors use a multiplexed FISH assay to monitor the 8 segments in parallel in infected cells suggesting bundling routes during the packaging process. |
| Databáze: | OpenAIRE |
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