Pharmacological characterization of volume-sensitive, taurine permeable anion channels in rat supraoptic glial cells
| Autor: | Brès, Vanessa, Hurbin, Amandine, Duvoid, Anne, Orcel, Hélène, C. Moos, Françoise, Rabié, Alain, Hussy, Nicolas |
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| Přispěvatelé: | Biologie des Neurones Endocrines [CNRS-UPR9055], Hurbin, Amandine, Biologie des neurones endocrines (BNE), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Male
osmoregula- tion Taurine [SDV]Life Sciences [q-bio] Gene Expression 4 4'-Diisothiocyanostilbene-2 2'-Disulfonic Acid Ion Channels Diffusion neuroendocrine cells Adenosine Triphosphate Chloride Channels Osmotic Pressure Animals ortho-Aminobenzoates RNA Messenger Rats Wistar hypothalamus In Situ Hybridization Cl 7 channel inhibitors volume-sensitive anion channels Dose-Response Relationship Drug Reverse Transcriptase Polymerase Chain Reaction Colforsin astrocytes Acetophenones Niflumic Acid Calcium Channel Blockers Rats [SDV] Life Sciences [q-bio] Tamoxifen Mibefradil Nitrobenzoates Papers Neuroglia Supraoptic Nucleus |
| Zdroj: | British Journal of Pharmacology British Journal of Pharmacology, Wiley, 2000, 130, ⟨10.1038/sj.bjp.0703492⟩ British Journal of Pharmacology, 2000, 130, ⟨10.1038/sj.bjp.0703492⟩ |
| ISSN: | 0007-1188 1476-5381 |
| Popis: | International audience; 1 To characterize the volume-sensitive, osmolyte permeable anion channels responsible for the osmodependent release of taurine from supraoptic nucleus (SON) astrocytes, we investigated the pharmacological properties of the [ 3 H]-taurine eux from acutely isolated SON. 2 Taurine release induced by hypotonic stimulus (250 mosmol l 71) was not antagonized by the taurine transporter blocker guanidinoethyl sulphonate, con®rming the lack of implication of the transporter. 3 The osmodependent release of taurine was blocked by a variety of Cl 7 channel inhibitors with the order of potency: NPPB4ni¯umic acid4DPC4DIDS4ATP. On the other hand, release of taurine was only weakly aected by other compounds (dideoxyforskolin, 4-bromophenacyl bromide, mibefradil) known to block volume-activated anion channels in other cell preparations, and was completely insensitive to tamoxifen, a broad inhibitor of these channels. 4 Although the molecular identity of volume-sensitive anion channels is not ®rmly established, a few genes have been postulated as potential candidates to encode such channels. We checked the expression in the SON of three of them, ClC 3 , phospholemman and VDAC 1 , and found that the transcripts of these genes are found in SON neurons, but not in astrocytes. Similar observation was previously reported for ClC 2. 5 In conclusion, the osmodependent taurine permeable channels of SON astrocytes display a particular pharmacological pro®le, suggesting the expression of a particular type or subtype of volume-sensitive anion channel, which is likely to be formed by yet unidenti®ed proteins. |
| Databáze: | OpenAIRE |
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