[Identification of Mycobacterium tuberculosis strains and a simultaneous identification of their drug resistance by the hybridization method on oligonucleotide microchips]

Autor:	Dmitry Gryadunov, Vm, Mikhaĭlovich, Sa, Lapa, Ni, Rudinskiĭ, Ve, Barskiĭ, Av, Chudinov, As, Zasedatelev, Ad, Mirzabekov
Předmět:	Bacterial Proteins Drug Resistance Bacterial Mutation Isoniazid Oligonucleotides Humans Mycobacterium tuberculosis Rifampin Catalase Oxidoreductases Oligonucleotide Array Sequence Analysis
Zdroj:	Europe PubMed Central
Popis:	A method of multiplex polymerase chain reaction (PCR) with subsequent hyoridization on oligonucleotide microchips was worked out to identify the Mycobacterium tuberculosis complex and to determine simultaneously the bacterial sensitivity to 2 first-line drugs, i.e. rifampin and isoniazid. The method provides for detecting above 95% of rifampin-resistant and around 80% of isoniazid-resistant strains within 1 day.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::ad5ef30a2a34588a753da89c90ee109c http://europepmc.org/abstract/med/14664159 Zobrazit plný text záznamu